Stop Losing Marks: Master the Maths in AQA Biology Paper 1/AS — Transcript
Full transcript
- 0:00Hey everyone and welcome to Miss Estric
- 0:02Biology and this entire math skills
- 0:04video for AS or Alevel paper 1 AQA
- 0:08biology. Now this isn't literally every
- 0:10single math skill that is on the math
- 0:12skills section of the spec. Instead what
- 0:14I've done is gone through the
- 0:16specification the theory part so topics
- 0:181 2 3 and four and wherever they say in
- 0:20the spec at this point this math skill
- 0:22could be assessed that is what I've
- 0:24picked out and gone through for this
- 0:25entire video. So, it's all of the topic
- 0:28related math skills that could come up.
- 0:30The topics 1 2 3 and four. And if you do
- 0:33want even more help on the math skills,
- 0:34then don't forget to check out my entire
- 0:38math skill workbook where it goes
- 0:39through every skill explained model
- 0:41example practice questions and the
- 0:44answers which I'll link in the
- 0:45description and the QR code just here.
- 0:48And keep a lookout for the paper 2 A
- 0:50level video that's coming and the entire
- 0:53set of math skills too. But for now,
- 0:55that's it. Let's jump into it. So let's
- 0:57go through the math skills that are
- 0:59specifically highlighted within the
- 1:01theory section for the AS or paper one
- 1:04for A level biology. So here's the first
- 1:06math skill that comes up for topic one
- 1:08in the specification calculating pH from
- 1:11hydrogen ion concentration using the
- 1:14formula.
- 1:17Now this exact math skill has never
- 1:20actually come up yet but elements of it
- 1:22have. So principles around the log and
- 1:26also hydrogen ions but literally
- 1:28calculating pH hasn't come up but let's
- 1:30just go through how to calculate pH from
- 1:33the hydrogen ion concentration using the
- 1:35formula in case it does come up or
- 1:37principles of it do come up as well.
- 1:40First of all pH is a measure of the
- 1:42hydrogen ion concentration in a
- 1:44solution. The more hydrogen ions there
- 1:46are the lower the pH. And that's why
- 1:48acids which release hydrogen ions have
- 1:51pH values lower than 7. The formula that
- 1:55we use is pH= minus log to the base 10
- 1:59of the hydrogen ion concentration. The
- 2:02square brackets around H+ just mean
- 2:05concentration and it must be in moles
- 2:08per decime cubed which we can see here.
- 2:11Now the log function here is base 10 not
- 2:15natural log. So, make sure you're
- 2:17pressing the correct button on your
- 2:19calculator.
- 2:21The minus sign is there. So, that a high
- 2:24hydrogen ion concentration gives a low
- 2:27pH and a low hydrogen ion concentration
- 2:31gives a high pH. So, that's what we mean
- 2:33by it flips the sense. It basically
- 2:36shows you high hydrogen ion
- 2:38concentration means low pH.
- 2:42So if we were to go through then an
- 2:45example and imagine that the hydrogen
- 2:49ion concentration is 3.2 * 10 ^ of - 5
- 2:54moles per decime cubed. First you need
- 2:56to type that number into your calculator
- 2:59and then press the log button. You
- 3:02should get -4.4949.
- 3:07Remember the formula says minus log so
- 3:10that we can take the negative of this
- 3:12value which gives us positive 4.4949.
- 3:17Finally we would round our answer
- 3:20because the concentration was given to
- 3:22us in two significant figures. We give
- 3:25the pH to two decimal places and that
- 3:28makes the pH equal to 4.5.
- 3:32And here we can see it step by step
- 3:34everything that you should get. You can
- 3:37also work backwards if you know the pH.
- 3:39The hydrogen ion concentration is 10 to
- 3:41the power of minus pH. So for example,
- 3:45if pH is 7.35,
- 3:48the concentration works out as 4.47
- 3:52* 10us 8.
- 3:58Next then we've got plot data from
- 4:00enzyme practicals in an appropriate
- 4:02graphical format. So the sorts of things
- 4:06you would need to do then is create a
- 4:07graph using the data that you're given
- 4:09or from your actual experiments and you
- 4:11need to know what to plot. So your
- 4:13independent variable always goes on the
- 4:15x-axis and that's actually the case for
- 4:17any graph and the independent variable
- 4:19is what you are deliberately changing.
- 4:23Dependent variable always goes on the y
- 4:25ais and that is what you are measuring.
- 4:28You always need to make sure that you're
- 4:30using the correct units and you put the
- 4:32units on your axes. And for enzyme
- 4:35experiments, it should always be a line
- 4:37graph, not a bar chart. So here we can
- 4:40see an example of one. And here's some
- 4:42more just general graph rules that
- 4:44they'd be looking for if they were
- 4:45asking you to draw a graph in the exam.
- 4:48So they would give you a section of
- 4:50graph paper. And you need to make sure
- 4:52that you pick a scale so that you fill
- 4:55at least half of the graph paper,
- 4:57whether that's in an exam or in your
- 4:59required practical. You also need to
- 5:01make sure that your scale goes up by
- 5:03even increments. So you've got an evenly
- 5:05spaced scale. You should be plotting
- 5:08your graph with small crosses. So each
- 5:10data point you put a cross for that
- 5:12position. And we should have this smooth
- 5:14curve line of best fit. And that could
- 5:16be a curve like we can see here. Or you
- 5:18might actually get a straight line of
- 5:20best fit. unlikely in an enzyme
- 5:22practical because we do have this
- 5:23increase up to an optimum and then it
- 5:25either plateaus or it will decrease. And
- 5:28then lastly, we don't tend to join the
- 5:32line together dot to dot between those
- 5:34data points unless you have so few data
- 5:37points that you can't accurately predict
- 5:40the line of best fit. But in the exam,
- 5:42it's unlikely they would give that to
- 5:43you. That's more relevant if you did the
- 5:45experiment yourself and you only had
- 5:47three data points, for example.
- 5:50Next then is using a tangent to
- 5:52calculate the initial rate of reaction
- 5:54from a graph. So reaction rates and this
- 5:56is all linking to the enzyme topic for
- 5:58topic one. Reaction rates are often
- 6:01determined by analyzing experimental
- 6:04data represented on a curved graph like
- 6:07we saw here. And when a reaction
- 6:10progresses the rate is usually changing
- 6:13over time. So to measure the rate of
- 6:15reaction at a specific point, we have to
- 6:18use a tangent to the curve and calculate
- 6:21the gradient of the tangent rather than
- 6:24that line of best fit. So let's go
- 6:27through an example then. So step one is
- 6:29to draw an usual tangent and we have to
- 6:31identify the point of interest. So in
- 6:34this case it might be they want to know
- 6:36the rate of reaction at 6 minutes. So
- 6:39you need to find 6 minutes on your
- 6:41graph. Next then you need to use a ruler
- 6:45to draw a straight line which will be
- 6:47our tangent that touches the curve at
- 6:50exactly the point that you chose. And
- 6:53then either side of where it touches the
- 6:56gap between your tangent and the curve
- 7:00should have equal angles. We can see
- 7:02here the gap between those two that
- 7:03angle there and that angle there is
- 7:06equal. So that's how you know the angle
- 7:08or the position to place your ruler to
- 7:11then draw that tangent.
- 7:14Now we can find the gradient of the
- 7:16tangent. So choose two clear points on
- 7:19the tangent. It doesn't actually matter
- 7:21anywhere on the tangent where you do
- 7:23this cuz it's a straight line. So no
- 7:25matter where you do this on the tangent,
- 7:26you'll get the same answer. But what
- 7:28we're going to work out is the change in
- 7:31y. Which means if we did this point and
- 7:34this point, we'd be reading off the
- 7:36value here and reading off the value
- 7:38here on our y axis and working out
- 7:40what's the difference between those. And
- 7:42we divide that between the change in x.
- 7:45So the time here, which is zero, and the
- 7:48time here, which is 9.6 minutes. And in
- 7:51fact, we can see all of that written
- 7:53here and worked out. So we then do the
- 7:56change in y / the change in x which is
- 7:58our gradient comes to 11.458 and here
- 8:02are our units mg per decime cubed which
- 8:05came from the concentration divided by
- 8:08time which is why it's per minute. So we
- 8:11then go on to using base frequencies to
- 8:14calculate the proportion of other bases
- 8:16in complimentary DNA strands. This is
- 8:18still from topic one and it's within the
- 8:20DNA section. So some key things just to
- 8:23be aware of to start with DNA base
- 8:25pairing rules. Adinine is always going
- 8:28to bind with thymine. They're
- 8:29complimentary base pairs. Cytosine will
- 8:31always bind with guanine. So that means
- 8:34in a double stranded DNA molecule,
- 8:36whatever percentage of adinine bases you
- 8:39have, you will have the same percentage
- 8:41of thymine because they always have to
- 8:42pair opposite each other. And the same
- 8:44with cytosine. Whatever percentage of
- 8:47cytosine bases you have, that'll be the
- 8:49same as the percentage of guanine. And
- 8:52because those are the only four bases
- 8:53you can have, the total of all of those
- 8:56percentages has to equal 100. So for
- 8:59this one, your method would be identify
- 9:02the percentage given in the question. So
- 9:04which of these bases do you have the
- 9:06percentage for? use the base pairing
- 9:08rule to find out the complimentary base
- 9:11and then you would take that away from
- 9:13100 and then half the rest between the
- 9:16other bases. But let me just show you an
- 9:17example of that. So we've got a DNA
- 9:19molecule contains 30% adinine bases.
- 9:23What are the proportion of the other
- 9:24three bases? Well, if we've got 30%
- 9:26adinine will have 30% thymine.
- 9:30Then we know that adinine and thymine
- 9:33are half of the bases. The other half is
- 9:35cytosine and guanine. So if 30 is
- 9:38adinine, 30 is thymine, then those
- 9:42together add up to 60. Take that away
- 9:44from 100 gives us 40% left. That has to
- 9:47be equally split between sides scene and
- 9:48guanine. So they must be 20% each. Next
- 9:52skill then is using the magnification
- 9:55formula. So magnification is image size
- 9:58divided by actual size. And you could
- 10:00have to rearrange that formula to work
- 10:02out the actual size of an object as
- 10:04well. unlikely they'd ask you to work
- 10:06out the image size because you would
- 10:08just look at the image and measure it
- 10:09with your ruler. So here's our formula
- 10:12again. And the size of the image is the
- 10:15size of the object in the actual
- 10:17microscope image. Whereas the size of
- 10:20the real object or actual size is the
- 10:24real life size of the structure you're
- 10:26looking at which is probably a cell or
- 10:28an organel in the cell. So we've got an
- 10:31example here. If the size of an image
- 10:34under the microscope is 200 micrometers,
- 10:38the actual size of the objects is 50
- 10:40micrometers and the magnification is
- 10:43this time we do 200 / 50 and that would
- 10:46tell us that the magnification is four
- 10:48times. So we can see just using that
- 10:50formula and the data that you were
- 10:52given. The units of measurements that
- 10:55you usually get are micrometers or
- 10:57nanometers for the actual size and it's
- 11:00normally actually micrometers because
- 11:03most of the organels in the cell are
- 11:06within the sizes that you would measure
- 11:09them in micrometers. We don't tend to
- 11:11use nanometers unless like it says here
- 11:14viruses are very very small. So you
- 11:16might have the units in nanometers. But
- 11:18when you're actually measuring it on
- 11:20your image size, you're likely going to
- 11:22be measuring in millimeters. But they
- 11:25usually ask you to give your final
- 11:27answer in micrometers. So you do have to
- 11:29do that conversion as well. And we'll
- 11:32see that in our worked example. So if we
- 11:34have a look at this one, we've got what
- 11:36is the magnification of the
- 11:39mitochondrian in your image? Show your
- 11:42working. So for this one, write out the
- 11:45formula. First of all, we've got
- 11:46magnification equals size of image
- 11:48divided by the size of the real object.
- 11:51The length of the image, if we were to
- 11:53measure it, it's been measured to be 84
- 11:56mm. And we've already been told that the
- 11:59actual length is 6 micrometers.
- 12:02So, we'd need to do 84 / 6. However,
- 12:06they have to be in the same units. and
- 12:0984 mm you can fit 1,000 microme into 1
- 12:15mm. So if we have 84 mm that means we
- 12:20have 84,000
- 12:22micrometers. So whenever you're
- 12:24converting your image size which will be
- 12:27in millime into micrometers you always
- 12:30times by a th00and as we then substitute
- 12:33in those values and we get 14,000. That
- 12:36is our magnification.
- 12:39The next math skill we've got is
- 12:41calculating the motic index from
- 12:43microscope slides. And here's the
- 12:45formula that you need to know for this
- 12:47one. The motic index is the number of
- 12:50cells that you can see in your
- 12:52microscope image in mitosis divided by
- 12:55the total number of cells. So what this
- 12:59calculation basically is is a measure of
- 13:01the proportion of cells undergoing
- 13:03mitosis and it's used to estimate how
- 13:06quickly a tissue is growing because if
- 13:08you have a higher motic index that
- 13:10indicates more cells are undergoing
- 13:11mitosis and therefore there must be more
- 13:13growth happening. So here's our formula.
- 13:16If we have a look at how you'd actually
- 13:18calculate this then you'd need to
- 13:20examine a microscope slide that shows
- 13:22dividing tissue. So for example, it
- 13:24might be an onion root tip field of view
- 13:27from your required practical two. You
- 13:30would then count the number of cells
- 13:32that you can see in any stage of
- 13:33mitosis. So whether it's prophase,
- 13:35metaphase, anaphase or telophase, count
- 13:38the total number of cells you can find
- 13:40in that field of view and then you'd use
- 13:43that formula number of cells in mitosis
- 13:45divided by a total number of cells. So,
- 13:47just to show you an example here, we've
- 13:49got a field of view that shows us some
- 13:53onion um root tip cells. And we can see
- 13:57if you to count them all, we've got 14
- 13:59cells. Four of them are in mitosis. So,
- 14:02we do 4 / 14. Our mitoic index is 0.29.
- 14:08Now, a few things just to point out.
- 14:09First of all, only ever count the whole
- 14:12cells. So, we can see here we've got a
- 14:14part of a cell. We've got a part of one
- 14:16there, a part there, there, there, and
- 14:19actually here as well. You wouldn't
- 14:21include those because if you can't see
- 14:23the whole cell, we can't actually see
- 14:25whether it's in mitosis or not. So only
- 14:27count on the whole cells. And the next
- 14:29thing is the way that we can tell if a
- 14:32cell is in mitosis or not is the
- 14:35chromosomes are visible. So this one
- 14:37here, we can see visible lines, which
- 14:39are the chromosomes. We can see visible
- 14:41lines in this one, this one, and this
- 14:42one. than in any particular
- 14:45organization. So it's probably prophase
- 14:48but we can see the chromosomes whereas
- 14:50here you don't see any lines. You don't
- 14:52see any chromosomes. Now the point I was
- 14:55making down here is this is just an
- 14:57image that I found um online but in the
- 15:00exam they would give you an image where
- 15:03it was really obvious where there was no
- 15:05debate about whether it is or is not in
- 15:09mitosis. It would be really obvious. You
- 15:11can see those lines. So just bear that
- 15:12in mind for the exam.
- 15:15Next we've got is using a scale bar to
- 15:17calculate the actual cell sizes. So in
- 15:21an magnified image like we can see here,
- 15:24sometimes you actually get a scale bar
- 15:26and from that you have to work out the
- 15:28magnification and they don't give you
- 15:30the magnification. And in this one we've
- 15:32got a mitochondrian and we've been given
- 15:34this scale bar and we're told that scale
- 15:37bar is 200 nm. Now what that means is
- 15:41that line there represents the actual
- 15:45size of 200 nmters. Not that if you were
- 15:49to look at that that is literally 200 nm
- 15:51on the image that is the actual size. So
- 15:54what we would then need to do is get our
- 15:56ruler and measure what is the image size
- 15:59of that line because we know the actual
- 16:02size is representing 200 nm and
- 16:06measuring this 16 mm. cuz obviously it
- 16:09depends what device you're looking at,
- 16:11but let's say it's 16 mm. You could then
- 16:14use your image and your actual to work
- 16:17out the magnification.
- 16:19So 1 millm again, we need to convert our
- 16:21units here. 1 mm is 1 million nanome. So
- 16:27you would need to do time 1 million cuz
- 16:31we've got 16 mm and there are 1 million
- 16:34nanometers in 1 millm. So we actually
- 16:37have 16 million nanometers.
- 16:40So we do 16 million / 200. And then that
- 16:44tells us the magnification of this image
- 16:47is 80,000 times.
- 16:50The next math skill is plotting results
- 16:53from permeability or osmosis
- 16:55experiments. And this could come up in
- 16:58your required practical three and four,
- 17:00the ones where you're using potato
- 17:03plants for osmosis or the permeability
- 17:05of the beetroot membranes. So for the
- 17:07osmosis one, your experiment could be
- 17:10with plant tissue such as potato
- 17:12cylinders in different sucrose or salt
- 17:14concentrations where you're going to be
- 17:16measuring a change in mass or length of
- 17:19your plant tissue. Then if you're
- 17:21plotting your results, if we go back to
- 17:23one of our earlier slides, we were
- 17:25talking about you always have your
- 17:26independent on the x-axis, your
- 17:28dependent on the y-axis. And the
- 17:30independent is what you're deliberately
- 17:32changing. And in this case, we'd be
- 17:34deliberately changing the concentration
- 17:36of the sucrose solution. So that would
- 17:38go on your xaxis. And we are measuring
- 17:41our dependent variable, the percentage
- 17:43change in mass. Or we'd actually measure
- 17:45the change in mass. So the initial and
- 17:48final mass work out what the change in
- 17:50mass is and then you always plot it as a
- 17:52percentage so it's comparable taking
- 17:54into account the fact that they might
- 17:55not have been exactly the same mass at
- 17:58the start and that goes on your y-axis
- 18:01the permeability experiments. So this
- 18:03would be your beetroot discs, maybe at
- 18:06different temperatures or in different
- 18:08solvents or different concentrations of
- 18:10solvent. And what you'd be measuring is
- 18:13how much pigment is in the solution
- 18:16after a set amount of time. And you
- 18:17could do that using a calimeter. So you
- 18:20get a numerical value or quantitative
- 18:23rather than just subject subjective
- 18:25describing how dark the purple color of
- 18:29the pigment from beetroot is. So in
- 18:32terms of plotting the data, our
- 18:33independent variable depends what's
- 18:35actually been varied. It might be the
- 18:37temperature, it might be the type of
- 18:38solvent, it might be the concentration
- 18:40of the solvent and that goes on your
- 18:42xaxis.
- 18:43The dependent variable is what you're
- 18:45measuring and we are measuring the
- 18:47amount of light being absorbed in that
- 18:49colorimeter or sometimes it's measured
- 18:51as the amount of light that transmits
- 18:52through. I always go for absorbance.
- 18:55So thinking about our graph rules, it
- 18:58would be a line graph cuz it's
- 18:59continuous data. We have to label the
- 19:01axes and we have to give the units as
- 19:04well. You have to use a suitable scale
- 19:07meaning your graph fits at least half of
- 19:10the paper and you have to increase by
- 19:12equal amounts on your scale. And lastly
- 19:15plotting your data points with an X and
- 19:17then you draw a line of best fit which
- 19:19is likely to be a smooth curve. So
- 19:22here's some example data. We've got our
- 19:24sucrose concentration and we've got the
- 19:26percentage change in mass and here is
- 19:30our graph. So we've got percentage
- 19:31change in mass, sucrossse concentration
- 19:34and there's our curved line of best fit.
- 19:37And this actually links to the next math
- 19:39skill determining the water potential
- 19:42from the intercept on a graph. So using
- 19:45that same concept we've got our sucrose
- 19:47concentration percentage change in mass
- 19:50and we've got our data plotted. the
- 19:52intercept. So this is where if we were
- 19:54to draw a dash line at where we have
- 19:57zero change in mass on our y axis that
- 20:00would be the water potential inside of
- 20:03the plant tissue which in this case is
- 20:05potato. And the reason for that is if
- 20:08you don't have any change in mass in an
- 20:10osmosis experiment that means there's
- 20:12been no net movement of water into the
- 20:16potato plant or out of the potato plant.
- 20:19And if there's no net movement of water
- 20:21basmosis, that tells us that the water
- 20:24potential inside of the potato tissue
- 20:27must be the same as the water potential
- 20:29of the sucrose solution. And that then
- 20:32indicates to us that at whatever point
- 20:36um the concentration is that we
- 20:38intercept at zero, that must mean that's
- 20:41the same concentration inside of the
- 20:44potato. instance, it tells us that the
- 20:47potato tissue must have a sucrose
- 20:49concentration of 0.4 cuz that is where
- 20:52the concentration is. Now, if you wanted
- 20:54to know what that was as a water
- 20:56potential, it's not literally just 0.4
- 20:59cuz that's a secret concentration, not a
- 21:01water potential concentration. So,
- 21:03sometimes there's an extra mark for
- 21:05saying you then need to look up in a
- 21:07table to see what water potential that
- 21:09concentration correlated to.
- 21:12The next skill, calculate the surface
- 21:15area to volume ratios of simple objects.
- 21:17So for example, cubes. So why does this
- 21:20matter? This is now topic three. Small
- 21:24organisms and cells exchange substances
- 21:27faster. So it links to the topic to do
- 21:29with gas exchange and absorption.
- 21:33Surf area to volume ratio shows how
- 21:36efficient an exchange surface is. So if
- 21:39you have a high surface area compared to
- 21:42the volume or surface area to volume
- 21:43ratio, that means you'll have faster
- 21:46diffusion or faster exchange relative to
- 21:49the size. And that could be diffusion of
- 21:51gases, absorption of substances, it
- 21:53could even be loss of heat across the
- 21:55surface. And if you have a lower surfer
- 21:58to volume ratio, you have a slower
- 22:00exchange system. And that is then when
- 22:02you start to see organisms have evolved
- 22:04over time and have these adaptations to
- 22:07increase exchange even though the entire
- 22:09organism has a low surfer to volume
- 22:12ratio. So for example gas exchange in
- 22:14the alvoli in the lungs or gas exchange
- 22:16in gills. So the formula for a cube
- 22:19would be working out the surface area
- 22:22which is 6 times because there's six
- 22:24faces of a cube the length squared
- 22:27because the area of one side of the cube
- 22:30is the length time the length and then
- 22:33there's six faces* 6. The volume of a
- 22:37cube would be the length cubed or in
- 22:39other words the height time the width
- 22:41time the length but because it's a cube
- 22:44those will all be the same dimension.
- 22:46And then it's whatever the surface area
- 22:48was divided by the volume. And we always
- 22:51present that as whatever the answer is
- 22:53to that calculation. We have that value
- 22:56to 1. So it's a ratio still. So we've
- 22:59got an example here. Cube with um
- 23:02dimensions of 1 cm. So 1 cm in height,
- 23:05length, and width. If we were to do our
- 23:07surface area, the length squared is
- 23:10still 1 * 6 cuz there's six faces to
- 23:14that cube. So that gives us 6 cm
- 23:16squared. The volume is the length cubed.
- 23:19So 1 * 1 * 1 which is 1. So 1 cm cubed.
- 23:24So that means our surface area divided
- 23:25by volume is 6 / 1. So we have a ratio
- 23:28of 6 to 1.
- 23:31Next then we have rearrange and use the
- 23:33formula for pulmonary ventilation rate
- 23:36which is pulmonary ventilation rate or
- 23:38PVR is tidal volume times breathing
- 23:40rate. So the PVR the pulmonary vol
- 23:43ventilation rate that is the volume of
- 23:46air ventilated per minute and that would
- 23:48typically be in decimeters cubed is our
- 23:51unit for volume per minute. Tidal volume
- 23:55is the volume of air per breath and
- 23:57because it's volume it's going to be in
- 23:58decubed and then breathing rate is
- 24:01number of breaths per minute. So that
- 24:04would be our formula. To rearrange it,
- 24:06if you wanted to find the tidal volume,
- 24:09it'd be your pulmonary ventilation rate
- 24:11divided by breathing rate. To work out
- 24:13the breathing rate, it would be
- 24:15pulmonary ventilation rate divided by
- 24:17the tidal volume. So, we've got a few
- 24:19examples. Then, a person has a tidal
- 24:22volume of 0.5 decimeters cubed and a
- 24:25breathing rate of 12 breaths per minute.
- 24:28What is the PVR? So this time it' be 0.5
- 24:31* 12 and that tells us the PVR is 6
- 24:34decimeters cubed per minute. Another
- 24:37example we've got a person has a PVR of
- 24:407.2 decime cubed per minute and a
- 24:43breathing rate of 18 breaths per minute.
- 24:45What's the tidal volume? So this time be
- 24:477.2 so PVR divided by the breaths per
- 24:51minute 18. So the volume the tidal
- 24:54volume is 0.4 decimeters cubed. Last
- 24:58option then a person has a tidal volume
- 25:00of 0.6 decimeters cubed and a PVR of 9
- 25:04decimeters cubed per minute. The
- 25:06breathing rate would be 9 for PVR
- 25:10divided by the tidal volume which is
- 25:120.6. So they have 15 breaths per minute.
- 25:18Math skill 12 on this list is from topic
- 25:21four using 2 to the^ of n to calculate
- 25:25the number of chromosome combinations
- 25:27and this links to meiosis where n is the
- 25:30number of pairs of chromosomes and the
- 25:32number of homologous pairs. So the
- 25:34principle here is that during meiosis
- 25:37chromosomes line up at the equator in
- 25:40independent segregation and then
- 25:42separate apart and which side of the
- 25:45equator the maternal and paternal
- 25:47chromosome align is random. So each
- 25:50homologous pair can arrange in two
- 25:52different ways either on the left or the
- 25:54right of the equator. So if we want to
- 25:57know how many possible different
- 25:58combinations there are that we could get
- 26:00in the gametes, it's two because we have
- 26:03pairs of chromosomes to the power of n
- 26:07where n is the number of pairs you have
- 26:08the number of homologous pairs. And in
- 26:11humans we have 23 homologous pairs of
- 26:14chromosomes. So that means 2 to the^ of
- 26:1723 which comes to over 8 million
- 26:20possible gameamt combinations that we
- 26:23can create before you even take into
- 26:26account crossing over and random
- 26:29fertilization of these gameamtes. So
- 26:31it's another way just to demonstrate how
- 26:34large the variation genetic variation is
- 26:37that is introduced from meiosis with a
- 26:39mathematical formula.
- 26:42Next then we've got interpret data using
- 26:44logarithmic scales and this often links
- 26:46to aseptic technique and growing
- 26:49bacteria because we use a log scale when
- 26:51you have a really large range of data.
- 26:55So many biological processes involve
- 26:58exponential growth and decay. So for
- 27:00example bacteria replication but we can
- 27:03also actually all see this in the growth
- 27:05of cancer cells. It comes up quite a bit
- 27:07in paper two the same skill. So
- 27:09logarithms help compare values that have
- 27:12a very large range. So comparing very
- 27:16small values to very large ones. And we
- 27:19can have logarithm to the base 10 or log
- 27:2310. And that is used for bacterial
- 27:25growth and dilution calculations.
- 27:28Natural logarithm or ln. That's mainly
- 27:30what we see. But you can get log e as
- 27:32well is applied in growth rate
- 27:34equations. But they'd normally give you
- 27:36an equation that you'd need to use if
- 27:38you had to do that. So just a bit more
- 27:41information on this. Here we can see
- 27:43some original values and this could be
- 27:47number of bacteria in a solution after a
- 27:49certain amount of time. And we can see
- 27:51here we've got data that is very very
- 27:54small going up to very very large. And
- 27:56it' be difficult to plot this on a graph
- 28:00so that you can actually get a scale
- 28:01that you could fit all of this on. And
- 28:03actually this probably isn't data from
- 28:05the number of bacteria because that's
- 28:06not a whole number and nor is that and
- 28:08you'd have to have a whole number for
- 28:09number of bacteria but it's
- 28:11demonstrating that concept. You've got a
- 28:13really really large range. So instead
- 28:16you'd have to convert your raw original
- 28:18values into log values log 10. So we can
- 28:22see here we've converted to log 10. And
- 28:25what that means is if you present your
- 28:26original value um in the order of
- 28:28magnitude value instead and this then
- 28:31gives us a much smaller range and we can
- 28:34then plot that on a graph. Now you can
- 28:38actually also get graph paper which is
- 28:42logarithmic graph paper and we can see
- 28:44here in this graph that's what we have.
- 28:46you don't have even
- 28:49subdivisions
- 28:51on this graph. And it's important that
- 28:54you know what each of those subdivisions
- 28:56means and how to read it off. So we can
- 28:59see here that where we've got 10^ the 2
- 29:03and that is the first line at 10^ the 2.
- 29:06So we've then got 1 2 3 4 5 and it
- 29:09actually goes up 10 divisions. The first
- 29:12line, the first point on 10^ the 2. What
- 29:14that means is 10 2 * 1 which is 100.
- 29:19This here is now the third line up
- 29:21because we've got that was line one,
- 29:23line two, line three. So that means it's
- 29:2610^ the 2 * 3 which is 300. This one
- 29:31here we've got 10 4. That's the first
- 29:33line. Second, third, fourth, fifth. So
- 29:37that's 10 4 * 5. And so on and so on. So
- 29:40that's how you read off on the y- axis
- 29:42on one of these graphs. Um so each of
- 29:45those lines is representing times
- 29:48whatever number line that is.
- 29:52Number 14 on our list is use and
- 29:55interpret the index of diversity formula
- 29:57which is from topic 4. So here is the
- 30:00formula. They usually give this to you
- 30:02in the exam but they don't often tell
- 30:03you what each component means. Sometimes
- 30:05they do but they don't always. So D is
- 30:08index of diversity and this is a measure
- 30:10of biodiversity taken into account
- 30:13species richness and also the number of
- 30:15individuals within the each of the
- 30:17populations of species. Capital N is the
- 30:21total number of organisms. So every
- 30:23living thing in that community
- 30:26lowerase N is the number of organisms of
- 30:29just one species. And that's why we have
- 30:32this symbol here, sum of, because this
- 30:35part you have to do for every species in
- 30:37your community. And then you add up all
- 30:40of those for all of your species. And
- 30:42that's normally where people go wrong on
- 30:44this formula. But if we go through um
- 30:46some examples,
- 30:48first of all, why do we actually do
- 30:50this? As I said, it's a measure of
- 30:51biodiversity in the community. The
- 30:53higher the index of diversity or D, that
- 30:56tells us we've got a greater
- 30:57biodiversity, which means we've got more
- 31:00species and more balanced population
- 31:02sizes in the species, meaning each
- 31:05species probably has a large population.
- 31:08A lower index of diversity means lower
- 31:11biodiversity. So, you might have fewer
- 31:13species or you might just have one
- 31:15species that dominates. So you might
- 31:17have 10 species present, but you've got
- 31:19a million individuals in one of those
- 31:23species and only five or two or very low
- 31:26number in all of the others and
- 31:27therefore they're at risk of going
- 31:28extinct. So it's not actually very high
- 31:31biodiversity.
- 31:33So here's an example. They wouldn't give
- 31:35you this column in the exam. They don't
- 31:37make it that easy. They tend to say
- 31:38here's your species. Here's the
- 31:40population size. So capital n is the
- 31:43total number of individuals in this
- 31:46community. So we'd need to add up that
- 31:48column and that comes to 50. For this
- 31:51bit we need to do the sum of lowerase n
- 31:54* lowerase n minus one. So we have to do
- 31:57that for all of the species present. And
- 32:00as I said they don't give you that
- 32:01column. So I just tend to add it on in
- 32:03the exam and draw it on myself. So this
- 32:06would be 20
- 32:09* 20 - 1. So 20 * 19. This would be 15 *
- 32:1414.
- 32:16This is 10 * 9. And this is 5 * 4.
- 32:20You're always doing n * n - 1. And then
- 32:23it's the sum of that column. And that
- 32:27comes to 700. So this numerator here
- 32:30then is the total number of individuals
- 32:33which was 50 * 50 - 1. So that is 50 *
- 32:3749 which is 2450
- 32:40divided by our denominator which was the
- 32:42sum of lowerase n * lowerase n minus one
- 32:47and it was a sum of all of those which
- 32:48was 700. So we've got an index of
- 32:51diversity overall value of 3.5. Now one
- 32:54thing just to try and gauge whether
- 32:56you've done this correctly using this
- 32:59exact formula you usually get an answer
- 33:02between 1 and 10. So if it's drastically
- 33:06over 10, you've gone wrong somewhere.
- 33:08And if it's lower than one, you've
- 33:10probably gone wrong. If it's lower than
- 33:12zero, you've definitely gone wrong.
- 33:14Next, then calculating mean values for a
- 33:17data set. So this one's quite
- 33:18straightforward. Mean is a measure of
- 33:20val of an average. So it's the sum of
- 33:22all the values divided by the number of
- 33:24values you have. So add up all of your
- 33:26repeats, divide by the number of repeats
- 33:28you had. So we've got an example here.
- 33:30So here's all the repeats. Add them all
- 33:33up and there were five repeats. So we
- 33:35divide by five. We've got a mean of
- 33:37nine. Now this goes hand inhand with
- 33:40standard deviation. And although it says
- 33:43on the spec calculate standard
- 33:44deviation, you might be asked to do that
- 33:46in your required practicals, but they
- 33:49won't ask you to do it in the exam
- 33:50because they do state it take up too
- 33:52many marks because of the time you'd
- 33:55need for it for the number of maths
- 33:57marks you're allowed. But you do
- 33:59interpret standard deviation and that
- 34:01comes up every single year. So when
- 34:03we're doing an experiment and we've
- 34:04collected the data, we usually calculate
- 34:06a mean. But it's important to do more
- 34:09than just calculate your average or
- 34:11mean. It's better to also do the
- 34:13standard deviation because that tells us
- 34:16how spread out all of those results are
- 34:19compared to your mean. So it gives us a
- 34:22better idea of the reliability, how
- 34:23spread out all of those data points are.
- 34:26So standard deviation compared to the
- 34:28range of data is better as well because
- 34:30the range just tells you the highest and
- 34:32the lowest. Standard deviation is
- 34:34considering all of your repeats. How
- 34:37spread out are they compared to the
- 34:38mean. So in calculating standard
- 34:40deviation, one standard deviation is
- 34:43taken into account 68% of the data and
- 34:47two times whatever your standard
- 34:49deviation is includes 95% of the data.
- 34:52And they normally give you that as a
- 34:54statement in the exam. They'll say
- 34:57standard deviation has been included and
- 34:59it's two times the standard deviation
- 35:00which includes 95% of the data. Now we
- 35:04don't need to get into the nitty-gritty
- 35:06of the maths behind that. What you need
- 35:08to know is that means for you that if
- 35:11when you take into account your standard
- 35:13deviations
- 35:15against the mean, if those values
- 35:17overlap, it tells us you do not have a
- 35:20significant difference between those two
- 35:22means. So that is the point of telling
- 35:25you that fact. So just a few other
- 35:27points that we've got here. The standard
- 35:29deviation shows the spread of data
- 35:30around the mean like we said whereas the
- 35:32range only shows you the highest and the
- 35:34lowest value. So it's not as useful. The
- 35:37standard deviation reduces the effect of
- 35:40anomalies because it's taking into
- 35:42account all of the data. Whereas the
- 35:44range includes anomalies. It's just
- 35:46showing you the highest and the lowest.
- 35:48So it can be quite skewed. And the
- 35:50standard deviation, this is the big
- 35:52thing that it's used for. It's used to
- 35:54indicate whether a difference between
- 35:55mean sets of data sets is significant or
- 35:58not. That's the main thing that is
- 36:01assessed on in the exam every year. So
- 36:03let's just focus on that and have a
- 36:05look. We've got an example of some data
- 36:07here. Two different types of treatment.
- 36:09Mean population size. Here's our mean.
- 36:11And we're told in brackets this is 2 *
- 36:13the standard deviation. So what you
- 36:16would need to do is from our highest
- 36:19value, highest mean 7.3. We'd need to
- 36:23minus 0.8.
- 36:25From our lower, which is 5.6, we need to
- 36:27add on 0.7. And then C. 5.6 + 0.7. Does
- 36:33that take the mean higher than 7.3 minus
- 36:370.8? And if it does, that means the
- 36:41standard deviations overlap. And if it
- 36:44doesn't, then that means they don't
- 36:46overlap. If you have an overlap, that
- 36:50would mean there is no significant
- 36:52difference between the means. Even
- 36:53though those numbers are different, 5.6
- 36:556 and 7.3 might be different. When you
- 36:58take into the stand take into account
- 36:59the standard deviations, they're not
- 37:01significantly different. You could also
- 37:04get it on a graph, which is easier to
- 37:06interpret cuz you can then just visibly
- 37:07see does this standard deviation line
- 37:11cross the same point on the y-axis as
- 37:13this one. And we can see here it
- 37:15doesn't. So that means we can visibly
- 37:17see those means are different. The
- 37:19standard deviation bars also don't
- 37:21overlap. So it tells us that there is a
- 37:24significant difference between those
- 37:26means. So hopefully you found this
- 37:29helpful. If you did find this helpful,
- 37:31then in my AQA math skill workbook, I
- 37:34have got every single math skill that is
- 37:37listed in the math skills section of the
- 37:39specification. In this workbook, you
- 37:42have an explanation of every math skill,
- 37:44a modeled example, practice questions,
- 37:46and all of the answers. And I'll link
- 37:48that in the description. But that is it
- 37:50for today's video. Look out for the
- 37:52paper 2 version and the entire set of
- 37:55skills still to come.
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