Microbiology: the Catalase test — Transcript

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1. 0:00today we're going to be doing the cataly
2. 0:02test and what it is is If an organism is
3. 0:05cataly positive it can break down
4. 0:08hydrogen peroxide into water and
5. 0:12oxygen so a cataly positive reaction is
6. 0:16going to cause bubbles whereas a catala
7. 0:19negative reaction is not going to have
8. 0:21any Bubbles at all so what I did was I
9. 0:25labeled the organisms out of the first
10. 0:28grouping of organisms we used in class
11. 0:31uh which are positive and those that are
12. 0:34negative in regard to gram positive coxy
13. 0:38so we have the stafilic caucus and the
14. 0:40micrococcus that should be positive and
15. 0:43the enterococus and the streptococus
16. 0:45which should be catalase negative now if
17. 0:48you look at the gram scheme of things
18. 0:50most everything is catalase positive
19. 0:53except for strp and
20. 0:55enterococus
21. 0:57um but in the first grouping
22. 1:01um we have the gram positive coxy gram
23. 1:03negative coxy and gra gram negative Coco
24. 1:07basili so that would be staf caucus
25. 1:10micrococcus enterococus and streptococus
26. 1:13are all Grand positive coxy then we have
27. 1:17neria which is gram negative coxy and um
28. 1:22moraxella querales which is the only
29. 1:25gram negative coxy in
30. 1:27moraxella and hemophilus which which is
31. 1:30gam negative cocoa basili so I put an
32. 1:34organism on each that should be POS one
33. 1:37that should be positive on here so I put
34. 1:39Sappo cacus arus on here and I put
35. 1:42streptococus a galactia on this one so
36. 1:45I'm going to add my hydrogen peroxide
37. 1:48and look for
38. 1:50bubbles okay so as expected the strap
39. 1:54tockus does not show any
40. 1:56bubbles on the video it looks really
41. 1:59white but there aren't bubbles whereas
42. 2:01on the staf lakus Arius look at all
43. 2:04those
44. 2:06bubbles okay so this is a positive test
45. 2:10here for staff and this is a negative
46. 2:14test here for strep