Good Microbiological Practices and Procedures (GMPP) 7: transport — Transcript

Full transcript

1. 0:05there will always be a need to transport
2. 0:08biological material from one place to
3. 0:10another this could be within a single
4. 0:14laboratory between laboratories in the
5. 0:18same facility or between facilities in
6. 0:22different locations safe methods and
7. 0:27procedures must be in place to prevent
8. 0:30any release and risk of infection to the
9. 0:32individuals transporting the materials
10. 0:35or anyone else the materials might come
11. 0:38into contact with
12. 0:45within the laboratory samples will often
13. 0:48need to be transported as part of normal
14. 0:50working practices for example moving
15. 0:53frozen cultures from the freezer to the
16. 0:56bench or culture vessels from a
17. 1:01biological safety cabinet to an
18. 1:03incubator it is important to have agreed
19. 1:06procedures in place to minimize the
20. 1:09likelihood of release or exposure all
21. 1:14vessels containing biological agents
22. 1:17should be clearly labeled with at least
23. 1:20the contents and date you should not
24. 1:27label agar plates on their lids if the
25. 1:30plates are dropped and the lids come off
26. 1:32you will not be able to identify the
27. 1:34samples left in the bases label agar
28. 1:37plates on the underside now if the
29. 1:40plates are dropped you will still be
30. 1:42able to identify them make sure lids are
31. 1:46secure
32. 1:47prior to transfer as an extra precaution
33. 1:51lids can be sealed with an adhesive film
34. 1:55plastic should be used where possible to
35. 1:59avoid a sharps hazard in case of
36. 2:01breakage purpose-made racks should be
37. 2:06used to transport bottles tubes or agar
38. 2:09plates safely moving items using a tray
39. 2:13will help to keep them secure and
40. 2:15contain any spillage when transporting
41. 2:21higher hazard biological agents it may
42. 2:24be preferable to place - Brax flasks or
43. 2:28plates in plastic boxes fitted with
44. 2:31lockdown lids the boxes should contain
45. 2:35absorbent material in case of a spill an
46. 2:39additional layer of containment could be
47. 2:41added where necessary for example items
48. 2:44could be placed in sealable plastic bags
49. 2:47along with absorbent material before
50. 2:50being put into the container
51. 2:53note that the outer containers should
52. 2:55also be clearly labeled with
53. 2:58at least the contents and date spill
54. 3:01kits should be available for use in the
55. 3:04event of a spill during transport and
56. 3:06laboratory personnel must be trained in
57. 3:09their use samples often need to be
58. 3:14transported between locations within the
59. 3:16same building for example from where
60. 3:19they are received and booked into the
61. 3:21system to the laboratory where they will
62. 3:25be processed or from a work area to a
63. 3:30separate storage room or between
64. 3:36laboratories for different types of
65. 3:38processing for example from microbiology
66. 3:41to biochemistry waste may also need to
67. 3:46be transported from where it is
68. 3:48generated to a different location for
69. 3:51decontamination or to a separate storage
70. 3:56area if samples are being transported in
71. 4:02boxes with lockable lids the outside of
72. 4:06the box should be clearly labeled and
73. 4:08surface decontaminated before it leaves
74. 4:11the laboratory there should be prior
75. 4:15communication with the intended
76. 4:17recipient of the materials when
77. 4:19transferring between departments so that
78. 4:22the recipient is aware that they will be
79. 4:24receiving the materials lack of
80. 4:29communication can lead to confusing
81. 4:31situations and wasted effort where many
82. 4:38or large samples are being transported
83. 4:40at the same time you should use a
84. 4:43trolley items should be stacked neatly
85. 4:45and ideally on one level trolleys which
86. 4:52are overloaded or badly organized are
87. 4:54more difficult to handle and give rise
88. 4:57to a much greater risk of items falling
89. 4:59off or spilling
90. 5:03a trolley with side panels or wire
91. 5:06baskets will provide more security for
92. 5:08larger loads transferring biological
93. 5:14agents between locations in the same
94. 5:16building must be planned organized and
95. 5:19carried out in a way that minimizes
96. 5:21transit through communal areas and
97. 5:23public thoroughfares samples may need to
98. 5:33be transported between buildings on the
99. 5:36same site all the precautions we've seen
100. 5:39so far will apply but further
101. 5:41consideration should be given to the
102. 5:43layers of packaging needed to minimize
103. 5:45the risk of leakage in transit this
104. 5:49could mean more layers of packaging with
105. 5:52enough absorbent material between layers
106. 5:54to absorb the full volume of infectious
107. 5:57material if it were to leak some plastic
108. 6:02packaging designed for specific
109. 6:04containers has absorbent material bonded
110. 6:07to its inner surface which can hold the
111. 6:09full volume of liquid in the container
112. 6:15it's recommended that the outermost
113. 6:18transport container is rigid and
114. 6:20impervious so that it can be easily
115. 6:23decontaminated this could be a locking
116. 6:27plastic storage box as we've seen
117. 6:29previously or a larger item such as a
118. 6:32cool box the package should be clearly
119. 6:36labeled with the sender the recipient
120. 6:38and the package contents the recipient
121. 6:45must be notified in advance of the
122. 6:47transfer personnel involved in the
123. 6:52transfer must be suitably trained on any
124. 6:55risks presented by the transfer and how
125. 6:58to safely reduce those risks
126. 7:09samples may be transported between
127. 7:12facilities in different parts of the
128. 7:14country or between countries using
129. 7:16postal or courier services in these
130. 7:20cases you must follow the UN
131. 7:22international model regulations on the
132. 7:25transport of infectious substances these
133. 7:28regulations govern how these samples are
134. 7:31packaged and labeled depending on the
135. 7:34biological material being transported
136. 7:36and give guidance on suitable packaging
137. 7:39methods for transport purposes
138. 7:42biological agents are classified into
139. 7:44three groups
140. 7:46category a infectious substances are
141. 7:49capable of causing permanent disability
142. 7:52life-threatening or fatal disease in
143. 7:55humans or animals the regulations
144. 7:58contain an indicative list of category a
145. 8:01pathogens but others not on the list may
146. 8:04meet the category a criteria
147. 8:07category a substances affecting humans
148. 8:11and animals are covered by UN 281 for
149. 8:14category a solid medical waste is
150. 8:17covered by UN three five four nine
151. 8:20category a substances affecting animals
152. 8:23only are covered by UN two nine double
153. 8:26zero
154. 8:28category B infectious substances are
155. 8:31capable of causing infection in
156. 8:33susceptible humans or animals but do not
157. 8:36meet the criteria for inclusion in
158. 8:38category a category B infectious
159. 8:42substances are covered by UN double
160. 8:44three seven three category B clinical or
161. 8:47medical wastes are covered by UN three
162. 8:50two nine one
163. 8:52samples in the exempt category are those
164. 8:56for which there is a minimal likelihood
165. 8:58that live biological agents are present
166. 9:00or environmental samples not considered
167. 9:03to be a significant hazard to health or
168. 9:06biological agents that are considered to
169. 9:08be non-pathogenic to animals and humans
170. 9:14the UN regulations also define packaging
171. 9:18systems which use packaging materials
172. 9:20capable of containing and adequately
173. 9:23absorbing the full volume of any leakage
174. 9:26should the primary containment be
175. 9:28breached a triple packaging system is
176. 9:32recommended for all three
177. 9:33classifications of infectious substances
178. 9:36and is required by the UN regulations
179. 9:39for many of them the primary receptacle
180. 9:42containing the biological agent must be
181. 9:45watertight leak proof and clearly
182. 9:48labeled the primary receptacle must be
183. 9:53wrapped in enough absorbent material to
184. 9:55absorb all fluid in case of a breakage
185. 9:58or leakage
186. 10:06the secondary packaging contains the
187. 10:08primary receptacle and must also be
188. 10:11watertight and leak proof the secondary
189. 10:19packaging may contain several primary
190. 10:21receptacles along with abs or burnt
191. 10:24material it should be noted that there
192. 10:28are volume and weight limits for
193. 10:30packaged infectious substances and these
194. 10:32should be checked before the final
195. 10:35package is made up the secondary
196. 10:39packaging must be surface decontaminated
197. 10:42before it is placed in the outer
198. 10:44packaging the third layer known as the
199. 10:49outer packaging is used to protect the
200. 10:52secondary packaging from physical damage
201. 10:54while in transit the outer packaging is
202. 10:57also used to display various information
203. 11:00including the contents the shipper and
204. 11:03recipient information UN certification
205. 11:06marks hazard symbols and orientation
206. 11:09arrows documentation that describes the
207. 11:14contents the identity of the shipper and
208. 11:16recipient and any other details required
209. 11:19by the UN model regulations must be
210. 11:22provided between the second and outer
211. 11:24layers of packaging for category a
212. 11:29substances the secondary and outer
213. 11:31layers should be rigid while for
214. 11:34category B these layers may be non rigid
215. 11:37such as plastic bags and envelopes you
216. 11:41should check through the regulations
217. 11:43carefully to make sure you are using the
218. 11:45right materials it's important to stress
219. 11:49that as well as the right packaging
220. 11:51material the correct information is
221. 11:54included with the package so that the
222. 11:56contents shipper and recipient can be
223. 11:59identified in case the package is
224. 12:01damaged mislaid or delivered to the
225. 12:04wrong address
226. 12:07in summary good practice for the
227. 12:11transport of biological agents includes
228. 12:14clear labeling on the outer surfaces of
229. 12:17containers or packaging where possible
230. 12:21use containers made of plastic rather
231. 12:24than glass minimize the likelihood of
232. 12:27spills by using racks trays lidded
233. 12:31containers and trolleys use absorbent
234. 12:35material between layers to absorb spills
235. 12:39surface decontaminate outer containers
236. 12:44have spill kits easily available avoid
237. 12:50transit through communal areas make sure
238. 12:54staff are adequately trained and
239. 12:56understand the need for clear
240. 12:58communication follow national and UN
241. 13:03model regulations and use the triple
242. 13:05packaging system where required
243. 13:12[Music]
244. 13:17you