Good Microbiological Practices and Procedures (GMPP) 6: workflow — Transcript

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1. 0:05the term workflow is used to describe
2. 0:09how the laboratory is set up in two
3. 0:11different work areas and the procedures
4. 0:14and systems that enable the people in
5. 0:16the facility to carry out their work
6. 0:18efficiently and safely it's important
7. 0:22that the workflow at the facility
8. 0:24reduces the risk of staff being exposed
9. 0:28to biological agents biological agents
10. 0:32being released outside the laboratory
11. 0:34and cross-contamination before any work
12. 0:39begins in a laboratory facility a
13. 0:42workflow plan should be in place the
14. 0:45plan should be discussed with staff so
15. 0:48that everyone understands their roles
16. 0:50and responsibilities and how the work
17. 0:52will be organized from receiving samples
18. 0:55through to reporting results laboratory
19. 1:00design and setup are important factors
20. 1:03for good workflow the equipment and
21. 1:06materials needed for particular tasks
22. 1:08should be close at hand and there needs
23. 1:11to be sufficient and well-organized
24. 1:13space so that staff can work without
25. 1:16getting in each other's way it's best
26. 1:20practice to have a one-way flow of work
27. 1:22for example an area at one end of the
28. 1:25laboratory to receive samples which
29. 1:28would lead into an area for initial
30. 1:30processing then on to further analysis
31. 1:33and checking of results and finally to
32. 1:36disposal of waste
33. 1:39it's important to note that different
34. 1:42processing procedures can have adverse
35. 1:44effects on each other for example the
36. 1:47cultivation of live biological agents
37. 1:50could contaminate samples being
38. 1:52processed for PCR potentially creating
39. 1:55false positive results so these types of
40. 1:58processes should not be carried out in
41. 2:00the same area within a laboratory
42. 2:05separation of conflicting activities may
43. 2:08be achieved by having designated areas
44. 2:10on work benches these could be marked
45. 2:13out using tape and labeled there are
46. 2:17some activities that should ideally be
47. 2:20carried out in separate laboratories or
48. 2:22rooms
49. 2:22examples include primary sample
50. 2:25collection media and some reagent
51. 2:30preparations molecular techniques such
52. 2:34as PCR cultivation of biological agents
53. 2:39and autoclaving where possible separate
54. 2:46reagents and equipment should be used to
55. 2:48avoid cross contamination
56. 2:50for example separate dedicated bottles
57. 2:53of a buffer solution should be used for
58. 2:56work involving live cultures and
59. 2:58molecular methods here a color coding
60. 3:01system is being used to make
61. 3:03identification easier ideally there
62. 3:07should be enough storage space on
63. 3:09shelving and in cupboards and drawers to
64. 3:12keep the working areas clear organize
65. 3:16the laboratory space so that any
66. 3:18regularly used items such as spare boxes
67. 3:22of pipette tips are stored in drawers or
68. 3:25cupboards near to the work area avoid
69. 3:29storing items on shelving above the
70. 3:32microbiology work area as any dust that
71. 3:35settles there can be dislodged and
72. 3:37contaminate the work surface floors
73. 3:41should not be used as storage areas and
74. 3:44should be kept clear of everything
75. 3:46except waste bins if they're needed
76. 3:50before starting work the workspace
77. 3:52should be clean and tidy if possible
78. 3:56keep the surface clear of everything
79. 3:58except the items needed to complete the
80. 4:01task at hand and keep these items
81. 4:03towards the back of the bench to ensure
82. 4:06you have enough room to work at the
83. 4:08front it's difficult to work well in a
84. 4:14messy environment it can link to
85. 4:17cross-contamination and accidents which
86. 4:19may expose laboratory staff to
87. 4:22biological agents work cannot flow
88. 4:27smoothly without the right equipment and
89. 4:29supplies there should be procedures in
90. 4:32place to make sure that equipment is
91. 4:34checked regularly and properly
92. 4:36maintained supplies such as PPE reagents
93. 4:46and stocks of biological agents and
94. 4:48chemicals must be stored correctly a log
95. 4:54and regular stock check must be in place
96. 4:56to ensure there are enough supplies to
97. 4:59do the work
98. 5:07before starting any work the information
99. 5:11received with the sample should be
100. 5:13checked to ensure it is present and
101. 5:15correct and has been logged into the
102. 5:17system the sample should be clearly
103. 5:20labeled to ensure it cannot be confused
104. 5:23with other samples where multiple
105. 5:28analyses are required on the same sample
106. 5:30the procedures must be carried out in
107. 5:33the right order and if any sub samples
108. 5:36need to be taken from the primary sample
109. 5:38this should be agreed before processing
110. 5:40starts before processing the sample make
111. 5:47sure that the workspace is available
112. 5:49clean and tidy and that reagents and
113. 5:52other supplies are prepared and close to
114. 5:55hand
115. 5:56sign-off may be required for certain
116. 5:58procedures prior to moving on to the
117. 6:01next phase of the work this will be
118. 6:03useful for tracking the journey of the
119. 6:05sample when you've finished the
120. 6:08procedure you should tidy up and clean
121. 6:11the area before moving on to the next
122. 6:13process to avoid confusion and misplaced
123. 6:17samples clear communication with
124. 6:20colleagues will be required before
125. 6:22sending the sample to a different
126. 6:24Department some samples may require
127. 6:28storage after processing some may go
128. 6:31straight to waste disposal and some may
129. 6:36be destroyed during sample processing
130. 6:38for example DNA extraction for molecular
131. 6:42analysis in any of these instances the
132. 6:48fate of the sample should be recorded so
133. 6:50that the sample journey through the
134. 6:52facility can be tracked where samples
135. 6:56need to be stored the type location and
136. 6:59duration of storage should be agreed and
137. 7:02recorded
138. 7:08analyzing and reporting results of tests
139. 7:11should be standardized to avoid
140. 7:13confusion and miscommunication data and
141. 7:17reports should be checked for potential
142. 7:20errors before they are provided to the
143. 7:22customer or service user data and
144. 7:26reports should also be stored for an
145. 7:29agreed period of time in case they need
146. 7:31to be revisited and for auditing
147. 7:34purposes good communications and
148. 7:39sufficient numbers of well-trained staff
149. 7:41are important factors at every stage of
150. 7:44the process from design and planning
151. 7:47through to delivering results if these
152. 7:50important provisions are not properly
153. 7:52implemented it's difficult to work
154. 7:54safely and produce reliable results in
155. 7:59summary good workflow includes a
156. 8:02workflow plan that details what work
157. 8:05will be carried out when and where it
158. 8:08will be done and who will be responsible
159. 8:12sufficient clean and tidy workspaces
160. 8:17designated rooms or segregated areas for
161. 8:21specific procedures appropriate storage
162. 8:25of consumables and equipment
163. 8:29a one-way flow of work to avoid
164. 8:32cross-contamination of samples
165. 8:35standardized methods sample tracking and
166. 8:42clear communication between staff
167. 8:51[Music]
168. 8:56you