CELL - THE UNIT OF LIFE | ENDOMEMBRANE SYSTEM , MITOCHONDRIA , PLASTIDS — Transcript
Full transcript
- 0:01So in this session we'll discuss the
- 0:04endommembrane system. In our previous
- 0:06session we have already discussed about
- 0:08the plasma membrane and the cell wall.
- 0:11So let us start with the cell organels.
- 0:15So in the among the cell organal we'll
- 0:18study some of the cell organels which
- 0:21works in a coordinated way. Okay. And
- 0:25these membrane bound organals constitute
- 0:30an endommembrane system. So what is
- 0:34endommembrane system? It is
- 0:39a group of
- 0:42membranous
- 0:46organels
- 0:51having
- 0:53coordinated function.
- 1:01Okay. So together they will function and
- 1:06a particular function
- 1:08is performed in a coordinated way and it
- 1:12includes their endopplasmic reticulum.
- 1:24Then goli complex,
- 1:27[snorts]
- 1:31lysosomes
- 1:36and fourth one is vacules.
- 1:40Okay. So let us uh uh discuss one by
- 1:43one. The first one is endopplasmic
- 1:46reticulum. So let me show you the
- 1:48picture. So here you can see that in
- 1:50this picture you can see here this is
- 1:54goli complex.
- 1:57Okay. Then here you can see that uh
- 2:01these is the these are the endopplasmic
- 2:05reticulum rough and smooth endopplasmic
- 2:08reticulum. So you can see that they are
- 2:10present very close to each other and
- 2:14besides that you can see that the lysogs
- 2:17are here. This is life. Soj. So they're
- 2:20very close to each other and they
- 2:23act or they function in a very
- 2:26particular uh that is coordinated
- 2:29manner. So let us first discuss the
- 2:31endopplasmic reticulum.
- 2:42Okay. The endopplasmic reticulum in
- 2:46short we can say it is ER. So these are
- 2:49the
- 2:54network of
- 2:59tiny tubular structures
- 3:08scattered in the cytoplasm.
- 3:18Okay. And uh this endopplasmic reticulum
- 3:24er divides the
- 3:32intracellular
- 3:34space
- 3:37into
- 3:40two compartments.
- 3:42Two
- 3:44compartments.
- 3:47So what are these two compartments?
- 3:50So the two compartments are uh let me
- 3:52add one more slide here.
- 3:58So the two compartments are
- 4:02the luminal
- 4:07that is inside
- 4:11endopplasmic reticulum. So as I said
- 4:14that endoplasmic reticulum is tubular
- 4:17structure. I'll show you the picture so
- 4:18that it will be very clear to you. And
- 4:20other one is extra lumininal
- 4:26that is cytoplasm.
- 4:34Okay. Now this endoplasmic kinetic I'll
- 4:38show you the picture. Okay. Let me show
- 4:40you the picture. So here you can see
- 4:42that uh this is the endopplasmic
- 4:47reticulum
- 4:49endoplasmic reticulum. You can see that
- 4:51it is attached to the nuclear membrane.
- 4:53This is nucleus. It is attached to
- 4:55nuclear membrane. So here it is it is
- 4:58forming a tubular branched structure. So
- 5:01this portion the inner portion is known
- 5:04as your lumininal luminal [snorts]
- 5:09portion or luminal part that is inside
- 5:11of the endopplasmic reticulum and this
- 5:14outer part is known as your cytoplasm or
- 5:18extra luminal
- 5:20portion. Okay. So now this
- 5:24endopplasmic reticulum
- 5:27is of two types
- 5:32that is all of you know you have studied
- 5:34it in class 9 rough and
- 5:39smooth [snorts]
- 5:41smooth er and rer.
- 5:44Now this uh what is this rough
- 5:46endopplasmic reticulum?
- 5:50The endopplasmic reticulum
- 5:54it bears ribosome
- 6:01on their surface.
- 6:09Okay. And this are frequently observed
- 6:12in the very important children. This
- 6:15point is re
- 6:19is frequently
- 6:24observed in the
- 6:31cells
- 6:35actively
- 6:38involved
- 6:41in protein synthesis.
- 6:48Why can you say because the ribosomes
- 6:51are the protein factory the actual site
- 6:53where the protein synthesis takes place.
- 6:56Okay. So [clears throat] this
- 6:59endomlasmic reticulum
- 7:02provides the surface for the ribosomes
- 7:05for protein synthesis. Unless and until
- 7:08the ribosomes are attached to the
- 7:10surface of the endopplasmic reticulum
- 7:13the protein synthesis cannot take place.
- 7:16So that's why the number of rough
- 7:18endopplasmic reticulum is more in the
- 7:21cells which is actively participating in
- 7:24a synthesis of a protein. Okay. And uh
- 7:29this uh
- 7:32what is another feature of this uh
- 7:34structure? The other feature is that the
- 7:37re here I'm writing the re or rough
- 7:42endoplasmic reticulum are extensive
- 7:49and continuous with
- 7:58the outer membrane of nucleus.
- 8:09So here in this picture you can see this
- 8:12is the nucleus. This is the nucleus and
- 8:16this is the outer membrane of nucleus
- 8:18and the endopplasmic reticulum is
- 8:21attached to the outer membrane of the
- 8:24nucleus. Okay. Now come to the other
- 8:26part
- 8:29of the type of
- 8:32uh endopplasmic reticulum that is smooth
- 8:34endopplasmic reticulum. So here the
- 8:36ribosomes are
- 8:41upset
- 8:44and it is the major site for
- 8:47it is the
- 8:50major site
- 8:54for synthesis of
- 8:58lipid
- 9:01very very important children I'll once
- 9:04again I'll surprise Summarize this one.
- 9:06So in animal cell
- 9:10in animal cell
- 9:15lipid like
- 9:19steroidal
- 9:25hormones
- 9:29are synthesized in
- 9:36s e r. Okay to
- 9:41r e r function. What is the function?
- 9:45Let me write down
- 9:48this is s e r.
- 9:52The function of RER is providing
- 9:59surface for
- 10:02protein synthesis.
- 10:06So it indirectly helps in synthesis of
- 10:10protein. But STR
- 10:13it is the site for it is the major site
- 10:16for
- 10:18site for
- 10:20synthesis of
- 10:23lipid.
- 10:25Okay. And besides that it al it also
- 10:28helps in
- 10:33detoxification.
- 10:38Okay. So here you can see that it is
- 10:41attached it is connected to the
- 10:43membrane. So any toxic material in this
- 10:46nucleus is uh uh is carried by this
- 10:50endoplasmic reticulum and then it is
- 10:53exit to outside. Okay. So it acts as a
- 10:56channel for the removal of the toxic
- 10:59substances.
- 11:01[snorts]
- 11:03Okay. Now come to the next organal that
- 11:06is goli
- 11:08apparatus.
- 11:16The golki apparatus these are also
- 11:19reticular structures.
- 11:25Renticular
- 11:30structures
- 11:31near nucleus.
- 11:36So near nucleus endoplasmic reticulum is
- 11:38also found because it is connected to
- 11:40the nuclear membrane and the golgi
- 11:42complexes are also found near the
- 11:45uh nucleus and it was first
- 11:50observed by
- 11:55camo
- 11:57goli.
- 12:00Actually in endopplasmic reticulum many
- 12:02other things are there uh structurally
- 12:04if we going to go to explain it it is
- 12:07having three components like system n
- 12:09tubule and uh vesicles but these are not
- 12:13there in your textbook so I have not
- 12:15discussed so um now come to
- 12:19golgei complex the scientist's name is
- 12:22Camilo Golgi in 1898
- 12:28and the golki Complex complex consists
- 12:32of
- 12:35flat
- 12:36it consist of the following structures
- 12:39that is sister A
- 12:44uh which is of 0.5 to 1
- 12:50micrometer
- 12:52and the system are flat
- 12:56and
- 12:58disapp it.
- 13:02Okay.
- 13:05And then number two uh next uh and these
- 13:08the system are
- 13:15parallel to each other.
- 13:23Okay. They are stacked over each other.
- 13:26And uh this system a
- 13:29Sister A
- 13:34are concentrically arranged with
- 13:46convex
- 13:49that is forming
- 13:53or C space.
- 13:55C space
- 13:58and concave.
- 14:03Just a minute. And concave
- 14:06that is your maturing face
- 14:14that is transface.
- 14:18Okay. So these
- 14:22C and transference are totally different
- 14:25but they are interconnected
- 14:28and besides that what else is present?
- 14:30So here I have shown you it is it
- 14:32consist of
- 14:36first one is cy then it is composed of
- 14:41vesicles
- 14:44and third one is vacules.
- 14:49Okay. Now let us come to the picture.
- 14:54So in this picture you can see that
- 14:57these are the
- 15:00system
- 15:02which are sack like and each sister has
- 15:05okay one more thing so this this is goli
- 15:09complex and this goli complex is present
- 15:12near the nucleus as well as here the
- 15:16endopplasmic reticulum is present. Okay
- 15:18and in one component of the endoplasmic
- 15:21reticulum is the vesicle. Okay. So these
- 15:25are suppose the vesicles. The vesicles
- 15:28are the packaged unit of the protein
- 15:31that is synthesized in the ribosome
- 15:33present on the surface of endoplasmic
- 15:35reticulum. Endoplasmic reticulum protein
- 15:39package vasicular form. Now e vicles
- 15:44these vesicles are the secrettory
- 15:46vesicles and these vesicles you can see
- 15:49that these vesicles are they are fused
- 15:56they are fused to form the
- 16:00sister they are forming the sister. So
- 16:03this has two part you can see that
- 16:06sister are stacking over each other
- 16:10and they are concentrically
- 16:14arranged. You can see concentrically
- 16:17arranged these are concentrically
- 16:18arranged. Now these vesicles containing
- 16:21the proteins now fused together to form
- 16:24the sack like
- 16:26so. So from this side the system is
- 16:29formed. So it is known as this is the
- 16:32convex part. So it is known as forming
- 16:35face. This is called forming face. And
- 16:37the other side is the mature face or the
- 16:42trans face. Okay. I think it is clear to
- 16:45you.
- 16:46So these are all system. Okay. And uh
- 16:50this is your you can see that this golgi
- 16:54apparatus has a that is your formic and
- 17:00maturic phase. Okay. Now let us come to
- 17:04the vacules. Okay. What are the vacules?
- 17:07You can see that from the um golgi
- 17:12complex some vacules are pinched off.
- 17:15The vacules are pinched off. So these
- 17:17pinched off vacules contains the
- 17:21processed protein. The protein that is
- 17:23synthesized in the endoplasmic reticulum
- 17:26comes to the goli complex. Then in the
- 17:30golgi complex they undergo processing
- 17:33and they form the
- 17:35um sul they may attempts or they may
- 17:40combine the protein with sulfate group
- 17:43aspect group or lipoprotein glyoprotein
- 17:47like this and then these are pinched off
- 17:49as the vacules. Okay. So so that is
- 17:53that's why this is the part of a
- 17:57the goli complex. Let let me write down
- 18:00the function
- 18:03function of
- 18:09goli apparatus.
- 18:14Okay. So it secretes m it is known as
- 18:16the secret house of the cell because it
- 18:20secretes
- 18:24materials to the
- 18:29intracellular
- 18:33target
- 18:35or
- 18:38outside the
- 18:42cell. Okay. So here what happens uh the
- 18:48ER from the ER the vesicles are released
- 18:53which is packaged with the
- 18:57so let me uh draw one flow chart flow
- 19:00chart so that it will be clear to you.
- 19:04[snorts]
- 19:05So endopplasmic reticulum which
- 19:07endopplasmic reticulum rough
- 19:09endopplasmic reticulum from the rough
- 19:12endopplasmic reticulum
- 19:15the vesicles
- 19:19are released and uh this vesicles
- 19:24contains the
- 19:29protein.
- 19:31Okay. Now this vesicle
- 19:35fuses with
- 19:40C space that is forming phase. Okay.
- 19:44Then moves towards the
- 19:51transpace that is
- 19:55mature face. Okay. And in this way the
- 20:00golgic apparatus and the endoplasmic
- 20:03reticulum remain in close association.
- 20:06Okay. So these
- 20:09proteins are so these proteins which is
- 20:11contained in the vesicles
- 20:14are processed.
- 20:18The proteins
- 20:22of vesicle
- 20:26are processed
- 20:31in the system
- 20:39and then they're released.
- 20:50at the
- 20:52transpace.
- 20:55Okay. So
- 20:59like glyoprotein,
- 21:03lipoprotein
- 21:09like this. Okay. So this is the function
- 21:12of goli complex and that's why it is
- 21:15known as secret house of the cell. Now
- 21:17come to the next organal
- 21:21that is lysoome.
- 21:28So this lossome are also membrane bound.
- 21:40Vasicular
- 21:45structure
- 21:46and formed by the
- 21:54process of
- 21:57packaging in the golapus
- 22:05in the Golgi.
- 22:10apparatus. So here one of the function
- 22:13of goli apparatus is also packaging. So
- 22:16how you see you can see here that this
- 22:21golgi apparatus the proteins contained
- 22:24in the vesicle the vesicles
- 22:27as it moves from the cease to transcend
- 22:30the protein in the vesicle gets
- 22:32processed. Okay. And it is packaged in
- 22:35the form of vesicles and some of these
- 22:38vesicle becomes the lysosome. And some
- 22:42of the vesicle containing the membrane
- 22:44protein and they fuse with the membrane
- 22:46and help in the formation of the
- 22:48membrane. Some packaged vesicles also
- 22:51come out of the cell. Okay. So that's
- 22:54why
- 22:55goli apparatus also helps in packaging.
- 22:59Okay. Now [snorts] the loal
- 23:06lizooal
- 23:09vesicle
- 23:12contain
- 23:15hydrolytic enzymes
- 23:25like a
- 23:28hydrolace. Yes.
- 23:32Um that means which will digest the
- 23:35various
- 23:37macroolelecules in presence of water.
- 23:40Hydroges like lipase
- 23:45which will digest the lipid. Then
- 23:48protease
- 23:51which will digest the protein. And then
- 23:53carbohydrate is
- 23:58which will digest the carbohydrate
- 24:02and uh these
- 24:04lysomes
- 24:06are also known as the suicidal
- 24:09bag.
- 24:13They called okay they digest
- 24:19the carbohydrates,
- 24:25proteins,
- 24:28lipids
- 24:31and nucleic acid.
- 24:35Okay. and they are known as the suicidal
- 24:42sack of the
- 24:45cell. Why? Because
- 24:49the old out cell
- 24:56is digested by
- 25:01the lysosome.
- 25:04And sometimes when the cell itself is
- 25:07nonfunctional
- 25:09then it is also digested by the
- 25:11lysosome. So here you can see in this
- 25:14picture. So this is the lysosome filled
- 25:16with the hydrolytic enzyme and you can
- 25:19see this lysosomes are very close to the
- 25:21goli apparatus. You can see from the
- 25:24golgi apparatus this dark color vesicles
- 25:27are arise and these vesicles becomes
- 25:30membrane bound and that forms the lyso
- 25:33zone. Okay, I think it is clear to you.
- 25:36Now come to the next endommembrance uh
- 25:39structure that is vacuum.
- 25:47Okay. So the vacules are the membrane
- 25:50bound
- 25:57spaces
- 25:59found in the
- 26:02cytoplasm.
- 26:07Okay. And uh it contains
- 26:13water
- 26:16cell sap
- 26:18excretory product
- 26:24and
- 26:26other materials.
- 26:28and other
- 26:31materials
- 26:33not useful for the cell. Not useful
- 26:38for the
- 26:41cell. So uh this is uh I am talking
- 26:45about the plant cell. So in plant cell
- 26:53a single
- 26:56large vele
- 27:00is present
- 27:03at the center.
- 27:08Okay. And uh this vacule is bounded by
- 27:19a single membrane
- 27:27called [snorts] a tonoplast.
- 27:31Very very important. Okay. This is
- 27:34called tonoplast. In plant cell about
- 27:3790%
- 27:38so 90% of the uh volume of the
- 27:43cell is occupied by the vacuum. Okay.
- 27:49Now this uh what is the function of
- 27:51tonoplast in plant cell? The donoplast
- 27:58facilitates
- 28:02the transport
- 28:07of ions
- 28:10and other materials.
- 28:17Yeah.
- 28:18against
- 28:21the concentration gradient
- 28:31[snorts]
- 28:32into
- 28:34the vacuum.
- 28:37Okay. So against the concentration
- 28:41gradient means active
- 28:44transport.
- 28:50Okay.
- 28:52So the uh so here how it works. Suppose
- 28:56this is the
- 28:58vacuum and this is the cell plant cell.
- 29:03Even though the concentration of any
- 29:05unused material is high here, even then
- 29:09the substance will enter into it. So
- 29:13here suppose a low is there still it
- 29:17enters into it which requires energy. So
- 29:19it is active transport. But in case of
- 29:23animals, so here you can see this is the
- 29:26structure of a vacule. Okay.
- 29:32uh this is shown in case of plant cell.
- 29:35Okay, this is the central vacule. And uh
- 29:39now come to the in case of animal cell.
- 29:43Uh in amoeba
- 29:49the contractile
- 29:50vacule
- 29:57has seen
- 29:59excretion
- 30:03and osmo regulation.
- 30:09Okay. And uh in many cells
- 30:14in many
- 30:18protests
- 30:20protest means what? Unicellular
- 30:26ukariots.
- 30:29In many in unisellular ukariots food
- 30:33vacules are formed
- 30:40are formed. Okay. So how these food
- 30:43vacules are formed? These food vacules
- 30:46are formed. Suppose
- 30:49let us take any protestant cell. So here
- 30:53it forms food cough.
- 30:56Okay. And the food particle is here.
- 30:58Then in the later stage
- 31:02it engulfves the food particle and here
- 31:05the food vacule is formed
- 31:09and this food vacule inside this food
- 31:12vacule the food is digested.
- 31:18Okay.
- 31:21So here you can see in this picture this
- 31:23is the plant cell where uh um that is
- 31:27[clears throat] a large portion of the
- 31:29vacule is taken up by the um vacu
- 31:33portion of the cell is taken up by the
- 31:34vacules and this is the in case of
- 31:38amieba the contractile veules is
- 31:41present. This contractile veule helps in
- 31:43excretion and balance of the electrolyte
- 31:46and the water that is osmo regulation.
- 31:49And here you can see this is paramesium
- 31:51which is the example of a protist cell
- 31:54where contractile vacule is present and
- 31:57besides that the food vacules are also
- 32:00present. Here you can see these are the
- 32:02food vacules. Okay. [sighs] Now next
- 32:06come to the next cell organal that is
- 32:08mitochondria.
- 32:13So mitochondria is not a part of
- 32:15endommembrane system. So these
- 32:18mitochondria are visible only when the
- 32:22cells are stained otherwise they are not
- 32:24visible their number
- 32:31shape
- 32:35and size
- 32:39per cell are variable
- 32:44and and it depends on the [cough]
- 32:49depending on physiological
- 32:58activity. So for example, let us take
- 33:00the human sperm. In human form, the
- 33:04middle piece,
- 33:10the middle piece
- 33:16has a spiral mitochondria.
- 33:23Okay, it has spiral mitochondria because
- 33:28a large amount of ATP can be so that
- 33:31large amount of large
- 33:36amount of
- 33:38ATP is synthesized and is required for
- 33:43the motility of the
- 33:47of the sperm. So this is one example how
- 33:51the safe the when the mitochondria is
- 33:53spiral then it surface area is increased
- 33:56so large number of ATP are produced.
- 34:00So it may be sausage set
- 34:09or cylindrical
- 34:14and uh its diameter is usually 0.2
- 34:19to 1.0 0 micrometer and uh its average
- 34:24size is 0.5 micrometer and length is
- 34:29this is diameter
- 34:33and length is 1.0 to 4.1 micrometer.
- 34:39This is the
- 34:41length. Okay. This mitochondria is a
- 34:46double membrane bound structure. It is a
- 34:57[snorts]
- 34:58double membrane bound structure
- 35:02with a outer membrane and inner
- 35:05membrane. So here you can see this is
- 35:06the inner membrane. This one is inner
- 35:10membrane.
- 35:12Okay, which is folded to form fingerlike
- 35:15projection. And this is the outer
- 35:17membrane. So what are these membranes?
- 35:20That is outer
- 35:24and inner
- 35:29membrane. Okay. [snorts]
- 35:32[gasps] And uh this
- 35:35membrane the inner membrane divides the
- 35:37lumen into two aquas compartment. So
- 35:41this membrane
- 35:44divides
- 35:47the lumen.
- 35:50Lumen means the portion that is covered
- 35:53by the membrane. lumen into
- 35:58two
- 36:02equas compartment.
- 36:10Okay. So outer compartment. So here you
- 36:14can see this is
- 36:17the outer compartment. So outer
- 36:21compartment
- 36:25and inner compartment.
- 36:34The inner compartment is known as
- 36:37matrix. Very very important. Okay. And
- 36:42[clears throat]
- 36:43the inner membrane as you can see here
- 36:46this this portion is the matrix. This
- 36:48portion is matrix. Okay. And the inner
- 36:53membrane is folded or it is projected
- 36:56into the matrix in the form of the
- 37:00crystal like structures.
- 37:02Okay. And these infoldings are known as
- 37:05Christe. Okay. So these infoldings are
- 37:09known as Christe. Here you can see these
- 37:12infoldings are known as Christe. Okay.
- 37:16Okay, just a minute. Let me add one more
- 37:19slide here.
- 37:24>> [snorts]
- 37:26>> So the inner membrane
- 37:31inner membrane
- 37:38forms a
- 37:41number of
- 37:46infoldings
- 37:50that is called
- 37:55very very important and it is presmed
- 37:58towards the
- 38:00matrix.
- 38:06Okay. And this Christ what is their
- 38:09function? Increase the
- 38:12[clears throat]
- 38:14surface area.
- 38:18Okay. And the two membranes have their
- 38:21own specific enzyme associated with the
- 38:24mitochondrial function. Outer membrane,
- 38:27inner membrane
- 38:29specific enzyme
- 38:32and they perform very very specific
- 38:34function. Okay. The matrix
- 38:41contains circular
- 38:45DNA,
- 38:48RNA
- 38:53and 70S ribosomes.
- 38:57It contains 70S ribosomes like that of
- 39:00the proariote and they together.
- 39:04So why it is present
- 39:08for synthesis of a protein. So that's
- 39:11why some components of a
- 39:16proteins are also found here.
- 39:20Okay. Now let us come to the function.
- 39:23Uh these christe the christriste are
- 39:26having here I will show you the picture.
- 39:28The christ is having the f0 f_sub_1
- 39:32particle. the stocked particles. Here
- 39:35you can see these are the stocked
- 39:38particles.
- 39:40So this portion this is the head
- 39:44this is
- 39:46base and this is the middle piece. Okay.
- 39:51Let me draw the diagram separately. So
- 39:56on the Christ
- 40:03So this is the this is known as this
- 40:06portion is f_sub_0 this is f_sub_1. So
- 40:09this is f0 f_sub_1 particles
- 40:17associated with
- 40:23the christ
- 40:27and they help in synthesis of ATP. So
- 40:31actually this head contains ATP synthes
- 40:38enzyme and have seen this [snorts] F0
- 40:42F_sub_1 particle has seen ATP synthesis
- 40:48and these ATP are the energy
- 40:53currency.
- 40:56Okay.
- 40:57So that's why it is known as powerhouse
- 41:01of the cell.
- 41:07Mitochondria is known as powerhouse of
- 41:09the cell. [cough]
- 41:12[clears throat]
- 41:13Now this mitochondria is also known as
- 41:18and there is a very special feature of
- 41:20about this. The mitochondria is
- 41:28is a semi
- 41:31autonomous
- 41:35organal.
- 41:38Why? See all the organels
- 41:41are under the control of the nucleus
- 41:47because
- 41:49but mitochondria
- 41:52because mitochondria
- 41:57has its own DNA
- 42:03and can
- 42:06synthesize. size.
- 42:11It's a own
- 42:14protein
- 42:17which can regulate
- 42:28some of your some of its
- 42:32activities.
- 42:38end
- 42:40partially
- 42:43under the control of
- 42:49nucleus.
- 42:51Okay. So its major function is synthesis
- 42:54of ATP. But ATP how ATP is synthesized?
- 42:59The ATP is synthesized during aerobic
- 43:01respiration. So ATP is synthesized here.
- 43:04So ATP synthesis takes place during
- 43:13respiration. Which respiration? Cellular
- 43:16respiration. When the respiration takes
- 43:18place at the cellular level. So this is
- 43:21all about
- 43:23mitochondria. Next come to the next cell
- 43:26organal that is
- 43:28the plastids.
- 43:35>> [snorts]
- 43:36>> So these are found
- 43:40only in
- 43:42plant cells.
- 43:46Okay. And uh [clears throat] and
- 43:49ucgenoids. Besides that they are found
- 43:52and they're also found in uglyoids.
- 43:58Okay. and uh they are very large in size
- 44:01and easily observable under the
- 44:03microscope and they contain
- 44:07various pigments. So based on the
- 44:13type of
- 44:16pigments
- 44:20it is of three types. What are they?
- 44:24chloroplast,
- 44:29chromoplast
- 44:34and lucoplast.
- 44:38Okay. So, chloroplast it contains the
- 44:41green pigment.
- 44:45Chromoplast contains colored pigments.
- 44:52And [snorts] this is lucoplast without
- 44:57pigment.
- 45:00[snorts]
- 45:01Okay. So here in this picture you can
- 45:04see these are the the chloroplast. It is
- 45:07green in color because of the presence
- 45:09of green pigment. Then chromoplast
- 45:12contains various colored pigments like
- 45:16your fcoanthine, picothine, pyosine. So
- 45:21different types of pigments are there
- 45:25and uh that's why they are colorful but
- 45:28this lucoplast you can see it is having
- 45:32no pigment so it is colorless. Okay. So
- 45:35these are the three types of plastids.
- 45:39Let us discuss
- 45:41in details in some [snorts] details
- 45:43about this. We'll discuss about the
- 45:46chloroplast in details later on. But uh
- 45:50chloroplasts
- 45:55it contains
- 46:00chlorophyll
- 46:04and
- 46:06carrot node.
- 46:13Okay. And what is their function? They
- 46:16trap energy
- 46:19for photosynthesis.
- 46:25Okay. Then come to chromoplast.
- 46:32The chromoplast contains
- 46:35fat soluble
- 46:39carateninoid pigments.
- 46:50like
- 46:53carotene,
- 46:57jantoil
- 47:03etc. Okay. And this gives
- 47:07red,
- 47:09yellow,
- 47:11orange color and other colors also.
- 47:14[snorts]
- 47:15And uh uh since they are having
- 47:19different colors, so they are present in
- 47:26leaves. Which type of leaves? Cru
- 47:29leaves.
- 47:31Fruits.
- 47:34that is ripened fruits
- 47:37and flowers.
- 47:40The next come to lucoplast.
- 47:45They don't have any
- 47:47pigment. They are colorless
- 47:53less and they're of various size and sap
- 47:56and their main function is storage.
- 48:00Their main function is storage. So basic
- 48:03on stories they are of three types
- 48:08aoplast.
- 48:14Okay. Stores starch
- 48:21or carbohydrate. Okay. Then elioplast
- 48:28it stores
- 48:30oil and fat.
- 48:34And the last one is aluroplast.
- 48:39It stores
- 48:44protein.
- 48:46Okay.
- 48:49Now next come to the chloroplast which
- 48:51we'll discuss in details. details.
- 48:55Okay.
- 48:57The chloroplast are
- 49:10okay. The chloroplast are double
- 49:13membrane bound organal
- 49:22like mitochondria.
- 49:24Okay. And they are found in
- 49:30meopil cells of leaf.
- 49:41Okay. And they may be they may be your
- 49:45lens set
- 49:49or they may be oval.
- 49:52They may be spherical.
- 49:56Okay.
- 49:57>> [snorts]
- 49:58>> And their number varies from
- 50:02number
- 50:04varies from
- 50:07algae to
- 50:12green algae to um that is in green algae
- 50:17from algae to meil.
- 50:23Okay. So in algae one may be present in
- 50:26meopil 20 to 40 per cell.
- 50:32So these algae if it is unisellular so
- 50:35this is also one per cell.
- 50:40Okay. Now [clears throat]
- 50:46now now come to the special feature of
- 50:47this chloroplast.
- 50:49The inner membrane of chloroplast.
- 51:00Chloroplast
- 51:01is less permeable.
- 51:10Okay. And the space of
- 51:14just a minute.
- 51:17Okay. Okay. Yeah,
- 51:25wait.
- 51:28Something went wrong.
- 51:32Let me go to this slide.
- 51:39Okay, now I reached here.
- 51:44[gasps]
- 51:46So the inner membrane of chloroplast is
- 51:51less permeable and the space limited by
- 51:55the inner membrane of chloroplast is
- 51:57called the space
- 52:02enclosed by
- 52:06inner membrane
- 52:08[snorts]
- 52:12is called a stroma.
- 52:15S T R O M A very important which is the
- 52:18matrix and this stroma contains
- 52:27a number of
- 52:32organized
- 52:37flattened
- 52:40membranous X
- 52:48called the thyloid.
- 52:54Okay, very very important children. And
- 52:56this uh thyloid encloses a space.
- 53:01Thyloid
- 53:05encloses
- 53:08a space
- 53:10called lumen. I will show you in the
- 53:13picture. [snorts] Okay. and the
- 53:17chlorophyll
- 53:23pigments
- 53:26are present in the
- 53:30philyloid.
- 53:32This is very important.
- 53:36Okay.
- 53:38So
- 53:40uh
- 53:43here in this picture you can see
- 53:46this is the stroma. This portion is the
- 53:49stroma. Okay red color. So these are the
- 53:53this is the portion of stroma. Okay
- 53:56matrix. And in the matrix you can see
- 53:58that the
- 54:01coin like structures are present and
- 54:04each coin like structure is known as
- 54:06thyopid.
- 54:09And the stacks of
- 54:14gran
- 54:15each stack is known as gran. Okay. So
- 54:20the philyloid contains the chlorophyll
- 54:23pigment which actually performs the
- 54:25photosynthes uh photosynthesis. Okay.
- 54:29Now
- 54:34the stacks of
- 54:38thyloid
- 54:44forms
- 54:50granom.
- 54:53Granam means singular plural is grana.
- 54:58Okay.
- 55:02Now uh in between the uh that is your uh
- 55:08granom
- 55:10there are
- 55:14flat membranous tibules.
- 55:27connects the connecting
- 55:32the
- 55:34philyloids
- 55:39is called stroma lamela is called stroma
- 55:45lamela. Okay, this is called stroma
- 55:48lamela.
- 55:50Now what are the other features of this
- 55:52one?
- 55:54Like [snorts] mitochondria. So let me
- 55:56show you. Okay, this is chloroplast.
- 55:58Suppose here are the philoids and they
- 56:02together form the gran. So here is
- 56:05another granom. Okay. And these coids
- 56:10are connected by
- 56:20okay. The philyloids are connected by
- 56:25tubular structures, flattened tubular
- 56:28structures and these structures are
- 56:30known as stroma
- 56:33lamela.
- 56:36Okay. And the stroma contains
- 56:47circular DNA
- 56:51like uh here is that present in the
- 56:53mitochondria.
- 56:54Then ribosomes,
- 57:01enzymes
- 57:03for synthesis of
- 57:08proteins and carbohydrates.
- 57:12Okay. And the ribosome is of 70s type.
- 57:1770s type ribosome is present. And
- 57:20besides that RNA is also present. And
- 57:24the chloroplast
- 57:29is also known as
- 57:36semi
- 57:39autonomous
- 57:43organal.
- 57:46Okay. And you know that it main function
- 57:48is
- 57:50photosynthesis. No need to say but in
- 57:54which part performs which physiological
- 57:58activities of the photosynthesis which
- 58:01reaction of photosynthesis that we'll
- 58:03discuss in the photosynthesis chapter.
- 58:07Okay. So today we have discussed the
- 58:10endommembrane system. Then the two major
- 58:13cell organels that is mitochondria and
- 58:16chloroplast
- 58:17that is plastids and among plastids
- 58:20chloroplast we have discussed. So what
- 58:22is the most important feature here? We
- 58:24have important things we learned here.
- 58:27The how these endommen systems are
- 58:30working in a coordinated manner and then
- 58:32these chloroplast and mitochondria are
- 58:35the semiautonomous organals because they
- 58:38have their own DNA. As a [snorts] result
- 58:41they can synthesize their own protein
- 58:43and they are self- reggulated to some
- 58:46extent but at the same time they are
- 58:49under the control of nucleus as well.
- 58:52Okay. So they are partially under the
- 58:54control of nucleus and partially
- 58:56self-control. That's why they are known
- 58:58as semiautonomous
- 59:00organels. Okay. So let us stop here. In
- 59:03the next session we'll discuss about the
- 59:06about other cell organels and the
- 59:08nucleus. Okay, till then, thank you.
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