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CELL - THE UNIT OF LIFE | ENDOMEMBRANE SYSTEM , MITOCHONDRIA , PLASTIDS — Transcript

by Little Spark · 4,294 words · 933 segments · language en · Watch on YouTube

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  1. 0:01So in this session we'll discuss the
  2. 0:04endommembrane system. In our previous
  3. 0:06session we have already discussed about
  4. 0:08the plasma membrane and the cell wall.
  5. 0:11So let us start with the cell organels.
  6. 0:15So in the among the cell organal we'll
  7. 0:18study some of the cell organels which
  8. 0:21works in a coordinated way. Okay. And
  9. 0:25these membrane bound organals constitute
  10. 0:30an endommembrane system. So what is
  11. 0:34endommembrane system? It is
  12. 0:39a group of
  13. 0:42membranous
  14. 0:46organels
  15. 0:51having
  16. 0:53coordinated function.
  17. 1:01Okay. So together they will function and
  18. 1:06a particular function
  19. 1:08is performed in a coordinated way and it
  20. 1:12includes their endopplasmic reticulum.
  21. 1:24Then goli complex,
  22. 1:27[snorts]
  23. 1:31lysosomes
  24. 1:36and fourth one is vacules.
  25. 1:40Okay. So let us uh uh discuss one by
  26. 1:43one. The first one is endopplasmic
  27. 1:46reticulum. So let me show you the
  28. 1:48picture. So here you can see that in
  29. 1:50this picture you can see here this is
  30. 1:54goli complex.
  31. 1:57Okay. Then here you can see that uh
  32. 2:01these is the these are the endopplasmic
  33. 2:05reticulum rough and smooth endopplasmic
  34. 2:08reticulum. So you can see that they are
  35. 2:10present very close to each other and
  36. 2:14besides that you can see that the lysogs
  37. 2:17are here. This is life. Soj. So they're
  38. 2:20very close to each other and they
  39. 2:23act or they function in a very
  40. 2:26particular uh that is coordinated
  41. 2:29manner. So let us first discuss the
  42. 2:31endopplasmic reticulum.
  43. 2:42Okay. The endopplasmic reticulum in
  44. 2:46short we can say it is ER. So these are
  45. 2:49the
  46. 2:54network of
  47. 2:59tiny tubular structures
  48. 3:08scattered in the cytoplasm.
  49. 3:18Okay. And uh this endopplasmic reticulum
  50. 3:24er divides the
  51. 3:32intracellular
  52. 3:34space
  53. 3:37into
  54. 3:40two compartments.
  55. 3:42Two
  56. 3:44compartments.
  57. 3:47So what are these two compartments?
  58. 3:50So the two compartments are uh let me
  59. 3:52add one more slide here.
  60. 3:58So the two compartments are
  61. 4:02the luminal
  62. 4:07that is inside
  63. 4:11endopplasmic reticulum. So as I said
  64. 4:14that endoplasmic reticulum is tubular
  65. 4:17structure. I'll show you the picture so
  66. 4:18that it will be very clear to you. And
  67. 4:20other one is extra lumininal
  68. 4:26that is cytoplasm.
  69. 4:34Okay. Now this endoplasmic kinetic I'll
  70. 4:38show you the picture. Okay. Let me show
  71. 4:40you the picture. So here you can see
  72. 4:42that uh this is the endopplasmic
  73. 4:47reticulum
  74. 4:49endoplasmic reticulum. You can see that
  75. 4:51it is attached to the nuclear membrane.
  76. 4:53This is nucleus. It is attached to
  77. 4:55nuclear membrane. So here it is it is
  78. 4:58forming a tubular branched structure. So
  79. 5:01this portion the inner portion is known
  80. 5:04as your lumininal luminal [snorts]
  81. 5:09portion or luminal part that is inside
  82. 5:11of the endopplasmic reticulum and this
  83. 5:14outer part is known as your cytoplasm or
  84. 5:18extra luminal
  85. 5:20portion. Okay. So now this
  86. 5:24endopplasmic reticulum
  87. 5:27is of two types
  88. 5:32that is all of you know you have studied
  89. 5:34it in class 9 rough and
  90. 5:39smooth [snorts]
  91. 5:41smooth er and rer.
  92. 5:44Now this uh what is this rough
  93. 5:46endopplasmic reticulum?
  94. 5:50The endopplasmic reticulum
  95. 5:54it bears ribosome
  96. 6:01on their surface.
  97. 6:09Okay. And this are frequently observed
  98. 6:12in the very important children. This
  99. 6:15point is re
  100. 6:19is frequently
  101. 6:24observed in the
  102. 6:31cells
  103. 6:35actively
  104. 6:38involved
  105. 6:41in protein synthesis.
  106. 6:48Why can you say because the ribosomes
  107. 6:51are the protein factory the actual site
  108. 6:53where the protein synthesis takes place.
  109. 6:56Okay. So [clears throat] this
  110. 6:59endomlasmic reticulum
  111. 7:02provides the surface for the ribosomes
  112. 7:05for protein synthesis. Unless and until
  113. 7:08the ribosomes are attached to the
  114. 7:10surface of the endopplasmic reticulum
  115. 7:13the protein synthesis cannot take place.
  116. 7:16So that's why the number of rough
  117. 7:18endopplasmic reticulum is more in the
  118. 7:21cells which is actively participating in
  119. 7:24a synthesis of a protein. Okay. And uh
  120. 7:29this uh
  121. 7:32what is another feature of this uh
  122. 7:34structure? The other feature is that the
  123. 7:37re here I'm writing the re or rough
  124. 7:42endoplasmic reticulum are extensive
  125. 7:49and continuous with
  126. 7:58the outer membrane of nucleus.
  127. 8:09So here in this picture you can see this
  128. 8:12is the nucleus. This is the nucleus and
  129. 8:16this is the outer membrane of nucleus
  130. 8:18and the endopplasmic reticulum is
  131. 8:21attached to the outer membrane of the
  132. 8:24nucleus. Okay. Now come to the other
  133. 8:26part
  134. 8:29of the type of
  135. 8:32uh endopplasmic reticulum that is smooth
  136. 8:34endopplasmic reticulum. So here the
  137. 8:36ribosomes are
  138. 8:41upset
  139. 8:44and it is the major site for
  140. 8:47it is the
  141. 8:50major site
  142. 8:54for synthesis of
  143. 8:58lipid
  144. 9:01very very important children I'll once
  145. 9:04again I'll surprise Summarize this one.
  146. 9:06So in animal cell
  147. 9:10in animal cell
  148. 9:15lipid like
  149. 9:19steroidal
  150. 9:25hormones
  151. 9:29are synthesized in
  152. 9:36s e r. Okay to
  153. 9:41r e r function. What is the function?
  154. 9:45Let me write down
  155. 9:48this is s e r.
  156. 9:52The function of RER is providing
  157. 9:59surface for
  158. 10:02protein synthesis.
  159. 10:06So it indirectly helps in synthesis of
  160. 10:10protein. But STR
  161. 10:13it is the site for it is the major site
  162. 10:16for
  163. 10:18site for
  164. 10:20synthesis of
  165. 10:23lipid.
  166. 10:25Okay. And besides that it al it also
  167. 10:28helps in
  168. 10:33detoxification.
  169. 10:38Okay. So here you can see that it is
  170. 10:41attached it is connected to the
  171. 10:43membrane. So any toxic material in this
  172. 10:46nucleus is uh uh is carried by this
  173. 10:50endoplasmic reticulum and then it is
  174. 10:53exit to outside. Okay. So it acts as a
  175. 10:56channel for the removal of the toxic
  176. 10:59substances.
  177. 11:01[snorts]
  178. 11:03Okay. Now come to the next organal that
  179. 11:06is goli
  180. 11:08apparatus.
  181. 11:16The golki apparatus these are also
  182. 11:19reticular structures.
  183. 11:25Renticular
  184. 11:30structures
  185. 11:31near nucleus.
  186. 11:36So near nucleus endoplasmic reticulum is
  187. 11:38also found because it is connected to
  188. 11:40the nuclear membrane and the golgi
  189. 11:42complexes are also found near the
  190. 11:45uh nucleus and it was first
  191. 11:50observed by
  192. 11:55camo
  193. 11:57goli.
  194. 12:00Actually in endopplasmic reticulum many
  195. 12:02other things are there uh structurally
  196. 12:04if we going to go to explain it it is
  197. 12:07having three components like system n
  198. 12:09tubule and uh vesicles but these are not
  199. 12:13there in your textbook so I have not
  200. 12:15discussed so um now come to
  201. 12:19golgei complex the scientist's name is
  202. 12:22Camilo Golgi in 1898
  203. 12:28and the golki Complex complex consists
  204. 12:32of
  205. 12:35flat
  206. 12:36it consist of the following structures
  207. 12:39that is sister A
  208. 12:44uh which is of 0.5 to 1
  209. 12:50micrometer
  210. 12:52and the system are flat
  211. 12:56and
  212. 12:58disapp it.
  213. 13:02Okay.
  214. 13:05And then number two uh next uh and these
  215. 13:08the system are
  216. 13:15parallel to each other.
  217. 13:23Okay. They are stacked over each other.
  218. 13:26And uh this system a
  219. 13:29Sister A
  220. 13:34are concentrically arranged with
  221. 13:46convex
  222. 13:49that is forming
  223. 13:53or C space.
  224. 13:55C space
  225. 13:58and concave.
  226. 14:03Just a minute. And concave
  227. 14:06that is your maturing face
  228. 14:14that is transface.
  229. 14:18Okay. So these
  230. 14:22C and transference are totally different
  231. 14:25but they are interconnected
  232. 14:28and besides that what else is present?
  233. 14:30So here I have shown you it is it
  234. 14:32consist of
  235. 14:36first one is cy then it is composed of
  236. 14:41vesicles
  237. 14:44and third one is vacules.
  238. 14:49Okay. Now let us come to the picture.
  239. 14:54So in this picture you can see that
  240. 14:57these are the
  241. 15:00system
  242. 15:02which are sack like and each sister has
  243. 15:05okay one more thing so this this is goli
  244. 15:09complex and this goli complex is present
  245. 15:12near the nucleus as well as here the
  246. 15:16endopplasmic reticulum is present. Okay
  247. 15:18and in one component of the endoplasmic
  248. 15:21reticulum is the vesicle. Okay. So these
  249. 15:25are suppose the vesicles. The vesicles
  250. 15:28are the packaged unit of the protein
  251. 15:31that is synthesized in the ribosome
  252. 15:33present on the surface of endoplasmic
  253. 15:35reticulum. Endoplasmic reticulum protein
  254. 15:39package vasicular form. Now e vicles
  255. 15:44these vesicles are the secrettory
  256. 15:46vesicles and these vesicles you can see
  257. 15:49that these vesicles are they are fused
  258. 15:56they are fused to form the
  259. 16:00sister they are forming the sister. So
  260. 16:03this has two part you can see that
  261. 16:06sister are stacking over each other
  262. 16:10and they are concentrically
  263. 16:14arranged. You can see concentrically
  264. 16:17arranged these are concentrically
  265. 16:18arranged. Now these vesicles containing
  266. 16:21the proteins now fused together to form
  267. 16:24the sack like
  268. 16:26so. So from this side the system is
  269. 16:29formed. So it is known as this is the
  270. 16:32convex part. So it is known as forming
  271. 16:35face. This is called forming face. And
  272. 16:37the other side is the mature face or the
  273. 16:42trans face. Okay. I think it is clear to
  274. 16:45you.
  275. 16:46So these are all system. Okay. And uh
  276. 16:50this is your you can see that this golgi
  277. 16:54apparatus has a that is your formic and
  278. 17:00maturic phase. Okay. Now let us come to
  279. 17:04the vacules. Okay. What are the vacules?
  280. 17:07You can see that from the um golgi
  281. 17:12complex some vacules are pinched off.
  282. 17:15The vacules are pinched off. So these
  283. 17:17pinched off vacules contains the
  284. 17:21processed protein. The protein that is
  285. 17:23synthesized in the endoplasmic reticulum
  286. 17:26comes to the goli complex. Then in the
  287. 17:30golgi complex they undergo processing
  288. 17:33and they form the
  289. 17:35um sul they may attempts or they may
  290. 17:40combine the protein with sulfate group
  291. 17:43aspect group or lipoprotein glyoprotein
  292. 17:47like this and then these are pinched off
  293. 17:49as the vacules. Okay. So so that is
  294. 17:53that's why this is the part of a
  295. 17:57the goli complex. Let let me write down
  296. 18:00the function
  297. 18:03function of
  298. 18:09goli apparatus.
  299. 18:14Okay. So it secretes m it is known as
  300. 18:16the secret house of the cell because it
  301. 18:20secretes
  302. 18:24materials to the
  303. 18:29intracellular
  304. 18:33target
  305. 18:35or
  306. 18:38outside the
  307. 18:42cell. Okay. So here what happens uh the
  308. 18:48ER from the ER the vesicles are released
  309. 18:53which is packaged with the
  310. 18:57so let me uh draw one flow chart flow
  311. 19:00chart so that it will be clear to you.
  312. 19:04[snorts]
  313. 19:05So endopplasmic reticulum which
  314. 19:07endopplasmic reticulum rough
  315. 19:09endopplasmic reticulum from the rough
  316. 19:12endopplasmic reticulum
  317. 19:15the vesicles
  318. 19:19are released and uh this vesicles
  319. 19:24contains the
  320. 19:29protein.
  321. 19:31Okay. Now this vesicle
  322. 19:35fuses with
  323. 19:40C space that is forming phase. Okay.
  324. 19:44Then moves towards the
  325. 19:51transpace that is
  326. 19:55mature face. Okay. And in this way the
  327. 20:00golgic apparatus and the endoplasmic
  328. 20:03reticulum remain in close association.
  329. 20:06Okay. So these
  330. 20:09proteins are so these proteins which is
  331. 20:11contained in the vesicles
  332. 20:14are processed.
  333. 20:18The proteins
  334. 20:22of vesicle
  335. 20:26are processed
  336. 20:31in the system
  337. 20:39and then they're released.
  338. 20:50at the
  339. 20:52transpace.
  340. 20:55Okay. So
  341. 20:59like glyoprotein,
  342. 21:03lipoprotein
  343. 21:09like this. Okay. So this is the function
  344. 21:12of goli complex and that's why it is
  345. 21:15known as secret house of the cell. Now
  346. 21:17come to the next organal
  347. 21:21that is lysoome.
  348. 21:28So this lossome are also membrane bound.
  349. 21:40Vasicular
  350. 21:45structure
  351. 21:46and formed by the
  352. 21:54process of
  353. 21:57packaging in the golapus
  354. 22:05in the Golgi.
  355. 22:10apparatus. So here one of the function
  356. 22:13of goli apparatus is also packaging. So
  357. 22:16how you see you can see here that this
  358. 22:21golgi apparatus the proteins contained
  359. 22:24in the vesicle the vesicles
  360. 22:27as it moves from the cease to transcend
  361. 22:30the protein in the vesicle gets
  362. 22:32processed. Okay. And it is packaged in
  363. 22:35the form of vesicles and some of these
  364. 22:38vesicle becomes the lysosome. And some
  365. 22:42of the vesicle containing the membrane
  366. 22:44protein and they fuse with the membrane
  367. 22:46and help in the formation of the
  368. 22:48membrane. Some packaged vesicles also
  369. 22:51come out of the cell. Okay. So that's
  370. 22:54why
  371. 22:55goli apparatus also helps in packaging.
  372. 22:59Okay. Now [snorts] the loal
  373. 23:06lizooal
  374. 23:09vesicle
  375. 23:12contain
  376. 23:15hydrolytic enzymes
  377. 23:25like a
  378. 23:28hydrolace. Yes.
  379. 23:32Um that means which will digest the
  380. 23:35various
  381. 23:37macroolelecules in presence of water.
  382. 23:40Hydroges like lipase
  383. 23:45which will digest the lipid. Then
  384. 23:48protease
  385. 23:51which will digest the protein. And then
  386. 23:53carbohydrate is
  387. 23:58which will digest the carbohydrate
  388. 24:02and uh these
  389. 24:04lysomes
  390. 24:06are also known as the suicidal
  391. 24:09bag.
  392. 24:13They called okay they digest
  393. 24:19the carbohydrates,
  394. 24:25proteins,
  395. 24:28lipids
  396. 24:31and nucleic acid.
  397. 24:35Okay. and they are known as the suicidal
  398. 24:42sack of the
  399. 24:45cell. Why? Because
  400. 24:49the old out cell
  401. 24:56is digested by
  402. 25:01the lysosome.
  403. 25:04And sometimes when the cell itself is
  404. 25:07nonfunctional
  405. 25:09then it is also digested by the
  406. 25:11lysosome. So here you can see in this
  407. 25:14picture. So this is the lysosome filled
  408. 25:16with the hydrolytic enzyme and you can
  409. 25:19see this lysosomes are very close to the
  410. 25:21goli apparatus. You can see from the
  411. 25:24golgi apparatus this dark color vesicles
  412. 25:27are arise and these vesicles becomes
  413. 25:30membrane bound and that forms the lyso
  414. 25:33zone. Okay, I think it is clear to you.
  415. 25:36Now come to the next endommembrance uh
  416. 25:39structure that is vacuum.
  417. 25:47Okay. So the vacules are the membrane
  418. 25:50bound
  419. 25:57spaces
  420. 25:59found in the
  421. 26:02cytoplasm.
  422. 26:07Okay. And uh it contains
  423. 26:13water
  424. 26:16cell sap
  425. 26:18excretory product
  426. 26:24and
  427. 26:26other materials.
  428. 26:28and other
  429. 26:31materials
  430. 26:33not useful for the cell. Not useful
  431. 26:38for the
  432. 26:41cell. So uh this is uh I am talking
  433. 26:45about the plant cell. So in plant cell
  434. 26:53a single
  435. 26:56large vele
  436. 27:00is present
  437. 27:03at the center.
  438. 27:08Okay. And uh this vacule is bounded by
  439. 27:19a single membrane
  440. 27:27called [snorts] a tonoplast.
  441. 27:31Very very important. Okay. This is
  442. 27:34called tonoplast. In plant cell about
  443. 27:3790%
  444. 27:38so 90% of the uh volume of the
  445. 27:43cell is occupied by the vacuum. Okay.
  446. 27:49Now this uh what is the function of
  447. 27:51tonoplast in plant cell? The donoplast
  448. 27:58facilitates
  449. 28:02the transport
  450. 28:07of ions
  451. 28:10and other materials.
  452. 28:17Yeah.
  453. 28:18against
  454. 28:21the concentration gradient
  455. 28:31[snorts]
  456. 28:32into
  457. 28:34the vacuum.
  458. 28:37Okay. So against the concentration
  459. 28:41gradient means active
  460. 28:44transport.
  461. 28:50Okay.
  462. 28:52So the uh so here how it works. Suppose
  463. 28:56this is the
  464. 28:58vacuum and this is the cell plant cell.
  465. 29:03Even though the concentration of any
  466. 29:05unused material is high here, even then
  467. 29:09the substance will enter into it. So
  468. 29:13here suppose a low is there still it
  469. 29:17enters into it which requires energy. So
  470. 29:19it is active transport. But in case of
  471. 29:23animals, so here you can see this is the
  472. 29:26structure of a vacule. Okay.
  473. 29:32uh this is shown in case of plant cell.
  474. 29:35Okay, this is the central vacule. And uh
  475. 29:39now come to the in case of animal cell.
  476. 29:43Uh in amoeba
  477. 29:49the contractile
  478. 29:50vacule
  479. 29:57has seen
  480. 29:59excretion
  481. 30:03and osmo regulation.
  482. 30:09Okay. And uh in many cells
  483. 30:14in many
  484. 30:18protests
  485. 30:20protest means what? Unicellular
  486. 30:26ukariots.
  487. 30:29In many in unisellular ukariots food
  488. 30:33vacules are formed
  489. 30:40are formed. Okay. So how these food
  490. 30:43vacules are formed? These food vacules
  491. 30:46are formed. Suppose
  492. 30:49let us take any protestant cell. So here
  493. 30:53it forms food cough.
  494. 30:56Okay. And the food particle is here.
  495. 30:58Then in the later stage
  496. 31:02it engulfves the food particle and here
  497. 31:05the food vacule is formed
  498. 31:09and this food vacule inside this food
  499. 31:12vacule the food is digested.
  500. 31:18Okay.
  501. 31:21So here you can see in this picture this
  502. 31:23is the plant cell where uh um that is
  503. 31:27[clears throat] a large portion of the
  504. 31:29vacule is taken up by the um vacu
  505. 31:33portion of the cell is taken up by the
  506. 31:34vacules and this is the in case of
  507. 31:38amieba the contractile veules is
  508. 31:41present. This contractile veule helps in
  509. 31:43excretion and balance of the electrolyte
  510. 31:46and the water that is osmo regulation.
  511. 31:49And here you can see this is paramesium
  512. 31:51which is the example of a protist cell
  513. 31:54where contractile vacule is present and
  514. 31:57besides that the food vacules are also
  515. 32:00present. Here you can see these are the
  516. 32:02food vacules. Okay. [sighs] Now next
  517. 32:06come to the next cell organal that is
  518. 32:08mitochondria.
  519. 32:13So mitochondria is not a part of
  520. 32:15endommembrane system. So these
  521. 32:18mitochondria are visible only when the
  522. 32:22cells are stained otherwise they are not
  523. 32:24visible their number
  524. 32:31shape
  525. 32:35and size
  526. 32:39per cell are variable
  527. 32:44and and it depends on the [cough]
  528. 32:49depending on physiological
  529. 32:58activity. So for example, let us take
  530. 33:00the human sperm. In human form, the
  531. 33:04middle piece,
  532. 33:10the middle piece
  533. 33:16has a spiral mitochondria.
  534. 33:23Okay, it has spiral mitochondria because
  535. 33:28a large amount of ATP can be so that
  536. 33:31large amount of large
  537. 33:36amount of
  538. 33:38ATP is synthesized and is required for
  539. 33:43the motility of the
  540. 33:47of the sperm. So this is one example how
  541. 33:51the safe the when the mitochondria is
  542. 33:53spiral then it surface area is increased
  543. 33:56so large number of ATP are produced.
  544. 34:00So it may be sausage set
  545. 34:09or cylindrical
  546. 34:14and uh its diameter is usually 0.2
  547. 34:19to 1.0 0 micrometer and uh its average
  548. 34:24size is 0.5 micrometer and length is
  549. 34:29this is diameter
  550. 34:33and length is 1.0 to 4.1 micrometer.
  551. 34:39This is the
  552. 34:41length. Okay. This mitochondria is a
  553. 34:46double membrane bound structure. It is a
  554. 34:57[snorts]
  555. 34:58double membrane bound structure
  556. 35:02with a outer membrane and inner
  557. 35:05membrane. So here you can see this is
  558. 35:06the inner membrane. This one is inner
  559. 35:10membrane.
  560. 35:12Okay, which is folded to form fingerlike
  561. 35:15projection. And this is the outer
  562. 35:17membrane. So what are these membranes?
  563. 35:20That is outer
  564. 35:24and inner
  565. 35:29membrane. Okay. [snorts]
  566. 35:32[gasps] And uh this
  567. 35:35membrane the inner membrane divides the
  568. 35:37lumen into two aquas compartment. So
  569. 35:41this membrane
  570. 35:44divides
  571. 35:47the lumen.
  572. 35:50Lumen means the portion that is covered
  573. 35:53by the membrane. lumen into
  574. 35:58two
  575. 36:02equas compartment.
  576. 36:10Okay. So outer compartment. So here you
  577. 36:14can see this is
  578. 36:17the outer compartment. So outer
  579. 36:21compartment
  580. 36:25and inner compartment.
  581. 36:34The inner compartment is known as
  582. 36:37matrix. Very very important. Okay. And
  583. 36:42[clears throat]
  584. 36:43the inner membrane as you can see here
  585. 36:46this this portion is the matrix. This
  586. 36:48portion is matrix. Okay. And the inner
  587. 36:53membrane is folded or it is projected
  588. 36:56into the matrix in the form of the
  589. 37:00crystal like structures.
  590. 37:02Okay. And these infoldings are known as
  591. 37:05Christe. Okay. So these infoldings are
  592. 37:09known as Christe. Here you can see these
  593. 37:12infoldings are known as Christe. Okay.
  594. 37:16Okay, just a minute. Let me add one more
  595. 37:19slide here.
  596. 37:24>> [snorts]
  597. 37:26>> So the inner membrane
  598. 37:31inner membrane
  599. 37:38forms a
  600. 37:41number of
  601. 37:46infoldings
  602. 37:50that is called
  603. 37:55very very important and it is presmed
  604. 37:58towards the
  605. 38:00matrix.
  606. 38:06Okay. And this Christ what is their
  607. 38:09function? Increase the
  608. 38:12[clears throat]
  609. 38:14surface area.
  610. 38:18Okay. And the two membranes have their
  611. 38:21own specific enzyme associated with the
  612. 38:24mitochondrial function. Outer membrane,
  613. 38:27inner membrane
  614. 38:29specific enzyme
  615. 38:32and they perform very very specific
  616. 38:34function. Okay. The matrix
  617. 38:41contains circular
  618. 38:45DNA,
  619. 38:48RNA
  620. 38:53and 70S ribosomes.
  621. 38:57It contains 70S ribosomes like that of
  622. 39:00the proariote and they together.
  623. 39:04So why it is present
  624. 39:08for synthesis of a protein. So that's
  625. 39:11why some components of a
  626. 39:16proteins are also found here.
  627. 39:20Okay. Now let us come to the function.
  628. 39:23Uh these christe the christriste are
  629. 39:26having here I will show you the picture.
  630. 39:28The christ is having the f0 f_sub_1
  631. 39:32particle. the stocked particles. Here
  632. 39:35you can see these are the stocked
  633. 39:38particles.
  634. 39:40So this portion this is the head
  635. 39:44this is
  636. 39:46base and this is the middle piece. Okay.
  637. 39:51Let me draw the diagram separately. So
  638. 39:56on the Christ
  639. 40:03So this is the this is known as this
  640. 40:06portion is f_sub_0 this is f_sub_1. So
  641. 40:09this is f0 f_sub_1 particles
  642. 40:17associated with
  643. 40:23the christ
  644. 40:27and they help in synthesis of ATP. So
  645. 40:31actually this head contains ATP synthes
  646. 40:38enzyme and have seen this [snorts] F0
  647. 40:42F_sub_1 particle has seen ATP synthesis
  648. 40:48and these ATP are the energy
  649. 40:53currency.
  650. 40:56Okay.
  651. 40:57So that's why it is known as powerhouse
  652. 41:01of the cell.
  653. 41:07Mitochondria is known as powerhouse of
  654. 41:09the cell. [cough]
  655. 41:12[clears throat]
  656. 41:13Now this mitochondria is also known as
  657. 41:18and there is a very special feature of
  658. 41:20about this. The mitochondria is
  659. 41:28is a semi
  660. 41:31autonomous
  661. 41:35organal.
  662. 41:38Why? See all the organels
  663. 41:41are under the control of the nucleus
  664. 41:47because
  665. 41:49but mitochondria
  666. 41:52because mitochondria
  667. 41:57has its own DNA
  668. 42:03and can
  669. 42:06synthesize. size.
  670. 42:11It's a own
  671. 42:14protein
  672. 42:17which can regulate
  673. 42:28some of your some of its
  674. 42:32activities.
  675. 42:38end
  676. 42:40partially
  677. 42:43under the control of
  678. 42:49nucleus.
  679. 42:51Okay. So its major function is synthesis
  680. 42:54of ATP. But ATP how ATP is synthesized?
  681. 42:59The ATP is synthesized during aerobic
  682. 43:01respiration. So ATP is synthesized here.
  683. 43:04So ATP synthesis takes place during
  684. 43:13respiration. Which respiration? Cellular
  685. 43:16respiration. When the respiration takes
  686. 43:18place at the cellular level. So this is
  687. 43:21all about
  688. 43:23mitochondria. Next come to the next cell
  689. 43:26organal that is
  690. 43:28the plastids.
  691. 43:35>> [snorts]
  692. 43:36>> So these are found
  693. 43:40only in
  694. 43:42plant cells.
  695. 43:46Okay. And uh [clears throat] and
  696. 43:49ucgenoids. Besides that they are found
  697. 43:52and they're also found in uglyoids.
  698. 43:58Okay. and uh they are very large in size
  699. 44:01and easily observable under the
  700. 44:03microscope and they contain
  701. 44:07various pigments. So based on the
  702. 44:13type of
  703. 44:16pigments
  704. 44:20it is of three types. What are they?
  705. 44:24chloroplast,
  706. 44:29chromoplast
  707. 44:34and lucoplast.
  708. 44:38Okay. So, chloroplast it contains the
  709. 44:41green pigment.
  710. 44:45Chromoplast contains colored pigments.
  711. 44:52And [snorts] this is lucoplast without
  712. 44:57pigment.
  713. 45:00[snorts]
  714. 45:01Okay. So here in this picture you can
  715. 45:04see these are the the chloroplast. It is
  716. 45:07green in color because of the presence
  717. 45:09of green pigment. Then chromoplast
  718. 45:12contains various colored pigments like
  719. 45:16your fcoanthine, picothine, pyosine. So
  720. 45:21different types of pigments are there
  721. 45:25and uh that's why they are colorful but
  722. 45:28this lucoplast you can see it is having
  723. 45:32no pigment so it is colorless. Okay. So
  724. 45:35these are the three types of plastids.
  725. 45:39Let us discuss
  726. 45:41in details in some [snorts] details
  727. 45:43about this. We'll discuss about the
  728. 45:46chloroplast in details later on. But uh
  729. 45:50chloroplasts
  730. 45:55it contains
  731. 46:00chlorophyll
  732. 46:04and
  733. 46:06carrot node.
  734. 46:13Okay. And what is their function? They
  735. 46:16trap energy
  736. 46:19for photosynthesis.
  737. 46:25Okay. Then come to chromoplast.
  738. 46:32The chromoplast contains
  739. 46:35fat soluble
  740. 46:39carateninoid pigments.
  741. 46:50like
  742. 46:53carotene,
  743. 46:57jantoil
  744. 47:03etc. Okay. And this gives
  745. 47:07red,
  746. 47:09yellow,
  747. 47:11orange color and other colors also.
  748. 47:14[snorts]
  749. 47:15And uh uh since they are having
  750. 47:19different colors, so they are present in
  751. 47:26leaves. Which type of leaves? Cru
  752. 47:29leaves.
  753. 47:31Fruits.
  754. 47:34that is ripened fruits
  755. 47:37and flowers.
  756. 47:40The next come to lucoplast.
  757. 47:45They don't have any
  758. 47:47pigment. They are colorless
  759. 47:53less and they're of various size and sap
  760. 47:56and their main function is storage.
  761. 48:00Their main function is storage. So basic
  762. 48:03on stories they are of three types
  763. 48:08aoplast.
  764. 48:14Okay. Stores starch
  765. 48:21or carbohydrate. Okay. Then elioplast
  766. 48:28it stores
  767. 48:30oil and fat.
  768. 48:34And the last one is aluroplast.
  769. 48:39It stores
  770. 48:44protein.
  771. 48:46Okay.
  772. 48:49Now next come to the chloroplast which
  773. 48:51we'll discuss in details. details.
  774. 48:55Okay.
  775. 48:57The chloroplast are
  776. 49:10okay. The chloroplast are double
  777. 49:13membrane bound organal
  778. 49:22like mitochondria.
  779. 49:24Okay. And they are found in
  780. 49:30meopil cells of leaf.
  781. 49:41Okay. And they may be they may be your
  782. 49:45lens set
  783. 49:49or they may be oval.
  784. 49:52They may be spherical.
  785. 49:56Okay.
  786. 49:57>> [snorts]
  787. 49:58>> And their number varies from
  788. 50:02number
  789. 50:04varies from
  790. 50:07algae to
  791. 50:12green algae to um that is in green algae
  792. 50:17from algae to meil.
  793. 50:23Okay. So in algae one may be present in
  794. 50:26meopil 20 to 40 per cell.
  795. 50:32So these algae if it is unisellular so
  796. 50:35this is also one per cell.
  797. 50:40Okay. Now [clears throat]
  798. 50:46now now come to the special feature of
  799. 50:47this chloroplast.
  800. 50:49The inner membrane of chloroplast.
  801. 51:00Chloroplast
  802. 51:01is less permeable.
  803. 51:10Okay. And the space of
  804. 51:14just a minute.
  805. 51:17Okay. Okay. Yeah,
  806. 51:25wait.
  807. 51:28Something went wrong.
  808. 51:32Let me go to this slide.
  809. 51:39Okay, now I reached here.
  810. 51:44[gasps]
  811. 51:46So the inner membrane of chloroplast is
  812. 51:51less permeable and the space limited by
  813. 51:55the inner membrane of chloroplast is
  814. 51:57called the space
  815. 52:02enclosed by
  816. 52:06inner membrane
  817. 52:08[snorts]
  818. 52:12is called a stroma.
  819. 52:15S T R O M A very important which is the
  820. 52:18matrix and this stroma contains
  821. 52:27a number of
  822. 52:32organized
  823. 52:37flattened
  824. 52:40membranous X
  825. 52:48called the thyloid.
  826. 52:54Okay, very very important children. And
  827. 52:56this uh thyloid encloses a space.
  828. 53:01Thyloid
  829. 53:05encloses
  830. 53:08a space
  831. 53:10called lumen. I will show you in the
  832. 53:13picture. [snorts] Okay. and the
  833. 53:17chlorophyll
  834. 53:23pigments
  835. 53:26are present in the
  836. 53:30philyloid.
  837. 53:32This is very important.
  838. 53:36Okay.
  839. 53:38So
  840. 53:40uh
  841. 53:43here in this picture you can see
  842. 53:46this is the stroma. This portion is the
  843. 53:49stroma. Okay red color. So these are the
  844. 53:53this is the portion of stroma. Okay
  845. 53:56matrix. And in the matrix you can see
  846. 53:58that the
  847. 54:01coin like structures are present and
  848. 54:04each coin like structure is known as
  849. 54:06thyopid.
  850. 54:09And the stacks of
  851. 54:14gran
  852. 54:15each stack is known as gran. Okay. So
  853. 54:20the philyloid contains the chlorophyll
  854. 54:23pigment which actually performs the
  855. 54:25photosynthes uh photosynthesis. Okay.
  856. 54:29Now
  857. 54:34the stacks of
  858. 54:38thyloid
  859. 54:44forms
  860. 54:50granom.
  861. 54:53Granam means singular plural is grana.
  862. 54:58Okay.
  863. 55:02Now uh in between the uh that is your uh
  864. 55:08granom
  865. 55:10there are
  866. 55:14flat membranous tibules.
  867. 55:27connects the connecting
  868. 55:32the
  869. 55:34philyloids
  870. 55:39is called stroma lamela is called stroma
  871. 55:45lamela. Okay, this is called stroma
  872. 55:48lamela.
  873. 55:50Now what are the other features of this
  874. 55:52one?
  875. 55:54Like [snorts] mitochondria. So let me
  876. 55:56show you. Okay, this is chloroplast.
  877. 55:58Suppose here are the philoids and they
  878. 56:02together form the gran. So here is
  879. 56:05another granom. Okay. And these coids
  880. 56:10are connected by
  881. 56:20okay. The philyloids are connected by
  882. 56:25tubular structures, flattened tubular
  883. 56:28structures and these structures are
  884. 56:30known as stroma
  885. 56:33lamela.
  886. 56:36Okay. And the stroma contains
  887. 56:47circular DNA
  888. 56:51like uh here is that present in the
  889. 56:53mitochondria.
  890. 56:54Then ribosomes,
  891. 57:01enzymes
  892. 57:03for synthesis of
  893. 57:08proteins and carbohydrates.
  894. 57:12Okay. And the ribosome is of 70s type.
  895. 57:1770s type ribosome is present. And
  896. 57:20besides that RNA is also present. And
  897. 57:24the chloroplast
  898. 57:29is also known as
  899. 57:36semi
  900. 57:39autonomous
  901. 57:43organal.
  902. 57:46Okay. And you know that it main function
  903. 57:48is
  904. 57:50photosynthesis. No need to say but in
  905. 57:54which part performs which physiological
  906. 57:58activities of the photosynthesis which
  907. 58:01reaction of photosynthesis that we'll
  908. 58:03discuss in the photosynthesis chapter.
  909. 58:07Okay. So today we have discussed the
  910. 58:10endommembrane system. Then the two major
  911. 58:13cell organels that is mitochondria and
  912. 58:16chloroplast
  913. 58:17that is plastids and among plastids
  914. 58:20chloroplast we have discussed. So what
  915. 58:22is the most important feature here? We
  916. 58:24have important things we learned here.
  917. 58:27The how these endommen systems are
  918. 58:30working in a coordinated manner and then
  919. 58:32these chloroplast and mitochondria are
  920. 58:35the semiautonomous organals because they
  921. 58:38have their own DNA. As a [snorts] result
  922. 58:41they can synthesize their own protein
  923. 58:43and they are self- reggulated to some
  924. 58:46extent but at the same time they are
  925. 58:49under the control of nucleus as well.
  926. 58:52Okay. So they are partially under the
  927. 58:54control of nucleus and partially
  928. 58:56self-control. That's why they are known
  929. 58:58as semiautonomous
  930. 59:00organels. Okay. So let us stop here. In
  931. 59:03the next session we'll discuss about the
  932. 59:06about other cell organels and the
  933. 59:08nucleus. Okay, till then, thank you.

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