Biological safety cabinet (BSC) 4: Incident management — Transcript

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1. 0:04biosafety cabinets are primary
2. 0:07containment devices designed to protect
3. 0:09laboratory workers and the surrounding
4. 0:11environment from potential exposure to
5. 0:13infectious agents in the event of an
6. 0:16emergency or cabinet malfunction
7. 0:18operators must be prepared to quickly
8. 0:20react in a safe and secure manner spills
9. 0:24can happen to the most seasoned
10. 0:26laboratory personnel standard operating
11. 0:28procedures for BSC use should therefore
12. 0:31include procedures for spill cleanups
13. 0:33inside of the SC with any spill inside
14. 0:37the biosafety cabinet a risk assessment
15. 0:40should be performed to determine the
16. 0:42severity of the event factors to
17. 0:44consider include the volume of material
18. 0:47spill the potential titer of the agent
19. 0:50involved if the spill is confined to a
20. 0:52small area or is spread over multiple
21. 0:55surfaces and whether your PPE may have
22. 0:58been contaminated in the process in the
23. 1:01event of a high-risk spill inside the
24. 1:03biosafety cabinet which can involve
25. 1:06large volumes multiple contaminated
26. 1:08surfaces high titers of infectious agent
27. 1:11or gross contamination of PPE the
28. 1:15following general procedures are
29. 1:16recommended a small amount of cleanup
30. 1:19related supplies should be kept inside
31. 1:21the cabinet during routine work contain
32. 1:25the spill if possible by covering it
33. 1:27with absorbent towel remove and discard
34. 1:30contaminated gloves within the BSC if
35. 1:33two pairs are worn discard the outermost
36. 1:36layer if sleeves are potentially
37. 1:39contaminated remove the lab coat or gown
38. 1:42and place into a bag for autoclaving
39. 1:45wash your hands and if necessary Don a
40. 1:49clean lab coat or gown put on a fresh
41. 1:53pair of gloves
42. 1:54allow any aerosols generated by the
43. 1:57spill to be removed by the cabinet's
44. 1:59HEPA filter this requires that the BSC
45. 2:02blower be left on and the sash remains
46. 2:05at the appropriate level to filter
47. 2:07aerosols generated by the spill
48. 2:09typically five minutes for most BSC s
49. 2:12during this time refrain from doing any
50. 2:15work in the cabinet take this
51. 2:17opportunity to prepare for the cleanup
52. 2:20gather any additional materials required
53. 2:23to deal with the spill and for the safe
54. 2:25removal of contaminated materials from
55. 2:27the cabinet choosing the most
56. 2:29appropriate disinfectant for the known
57. 2:31or suspected agent you're working with
58. 2:33should be part of your risk assessment
59. 2:35as a general recommendation sodium
60. 2:38hypochlorite commonly known as bleach is
61. 2:40an effective broad-spectrum disinfectant
62. 2:43when used at a concentration of 5000 ppm
63. 2:46higher concentrations may be required if
64. 2:50deemed necessary as per the risk
65. 2:52assessment after the aerosols have
66. 2:55cleared cover the spill with disposable
67. 2:57cloth or paper towels if not already
68. 2:59performed starting at the outer margin
69. 3:02of the spill area and working in a
70. 3:04circular motion towards the centre pour
71. 3:07disinfectant over the absorbent paper
72. 3:09and the immediately surrounding area
73. 3:11leave the soaked paper on top of the
74. 3:14spill for the appropriate contact time
75. 3:16in the meantime surface decontaminate
76. 3:19any reusable supplies using an absorbent
77. 3:22paper soaked with disinfectant
78. 3:24if material has spilled through the
79. 3:27grille of the BSC pour disinfectant
80. 3:29through the grille to flood the catch
81. 3:31tray underneath ensure that the drain
82. 3:34valve is closed once sufficient time has
83. 3:37passed clear away the towels and debris
84. 3:41if broken glass or other sharps are
85. 3:43involved use forceps to collect and
86. 3:46deposit the material into a puncture
87. 3:48resistant container for disposal
88. 3:51everything must be disposed of in an
89. 3:53appropriately labeled biohazard bag or
90. 3:56container inside the BSC clean and
91. 4:00disinfect the area of the spillage again
92. 4:02by wiping with a fresh paper towel
93. 4:04soaked with disinfectant if any liquid
94. 4:07has passed through the grille raise the
95. 4:10work surface open the drain valve to
96. 4:12remove excess liquid clean the catch
97. 4:15tray and then replace the work surface
98. 4:19spray the full interior of the cabinet
99. 4:22with disinfectant and wipe down as
100. 4:24appropriate if sodium hypochlorite was
101. 4:27used for disinfection make sure the
102. 4:29cabinet is further wiped down with
103. 4:31sterile water or 70% ethanol to avoid
104. 4:34corrosion allow BSC to run for at least
105. 4:3810 minutes before resuming work or
106. 4:41shutting down if the spill is deemed to
107. 4:44be minor the cleanup procedure will
108. 4:47follow similar steps to that of a major
109. 4:49spill but you may not be required to
110. 4:51leave the biosafety cabinet in this case
111. 4:54contain the spill with absorbent paper
112. 4:56soaked with disinfectant leave the towel
113. 4:59undisturbed for the disinfectants
114. 5:01appropriate contact time when the
115. 5:03appropriate contact time has passed
116. 5:05follow the steps as discussed before
117. 5:08biosafety cabinets require a constant
118. 5:11power supply to function correctly as a
119. 5:14primary containment device a sudden loss
120. 5:17of power to the biosafety cabinet
121. 5:19requires laboratory personnel to take
122. 5:21quick action to minimize the likelihood
123. 5:24of escape of biological material from
124. 5:26the biosafety cabinet remember not to
125. 5:29panic if cabinet power is lost stop your
126. 5:32work immediately
127. 5:34secure any infectious material by
128. 5:36capping tubes covering plates and
129. 5:39closing any open bottles disinfect and
130. 5:42remove gloves inside the BSC remove
131. 5:46hands from the cabinet close the sash
132. 5:48and leave the area until the power goes
133. 5:51back on notify others in the area of
134. 5:54potential exposure to biological
135. 5:56materials and place signage on cabinet
136. 5:59indicating it is out of order once power
137. 6:02returns reopen the sash and allow five
138. 6:05minutes for the air to purge and
139. 6:06stabilize before recommencing work if
140. 6:09power is not reestablished wait 30
141. 6:12minutes for possible aerosols to settle
142. 6:14Don additional PPE as required by your
143. 6:18local risk assessment open the sash and
144. 6:21surface decontaminate the BSC and all
145. 6:24work items prior to their removal
146. 6:26allowing for sufficient contact time do
147. 6:29not attempt to use the biosafety cabinet
148. 6:32until the issue has been resolved a
149. 6:34highly useful tool to reduce the risk of
150. 6:37power loss of a BSc is an
151. 6:39uninterruptible power supply or UPS a
152. 6:42UPS provides equipment with a limited
153. 6:45amount of continuous energy when there
154. 6:47is a periodic instability in the
155. 6:49building's energy supply a UPS can
156. 6:52provide staff working at a BSc with
157. 6:55enough time to properly secure
158. 6:56infectious material disinfect cabinet
159. 6:59surfaces and other items and
160. 7:01appropriately segregate waste materials
161. 7:04in a quick but safe manner generally
162. 7:07speaking a UPS capable of providing a
163. 7:09BSc with 10 minutes of uninterruptible
164. 7:12power should provide enough time for a
165. 7:14worker to safely shut down the BSC
166. 7:16before power is lost consult the
167. 7:19manufacturers specification for your
168. 7:21cabinets electrical requirements to
169. 7:23select the most appropriate UPS for your
170. 7:26needs there are other unexpected
171. 7:29instances or events such as a medical
172. 7:32emergency building alarm fire or other
173. 7:34immediate threat where an operator may
174. 7:37need to suddenly stop their work if safe
175. 7:40to do so
176. 7:41cure any infectious material within the
177. 7:43BSC remove PPE as per standard protocol
178. 7:47and exit the laboratory however the most
179. 7:50important thing to remember in any
180. 7:52emergency situation is that personal
181. 7:54safety comes first after any incident
182. 7:58it's important for laboratory staff to
183. 8:00report the situation to their supervisor
184. 8:02or safety officer as soon as possible
185. 8:05this provides the laboratory an
186. 8:07opportunity to identify potential causes
187. 8:09of the incidents and to come up with
188. 8:12ways to reduce their likelihood in the
189. 8:14future also it ensures that any persons
190. 8:17involved will receive appropriate
191. 8:18medical surveillance if deemed necessary
192. 8:21let's conclude this video with a review
193. 8:24of the following key messages a facility
194. 8:27standard operating procedures should
195. 8:29include protocols for unexpected
196. 8:31circumstances while working in a BSC
197. 8:34spills should be promptly contained and
198. 8:36decontaminated using an appropriate
199. 8:38disinfectant for the specified contact
200. 8:41time leave the cabinet running and
201. 8:44dispose of all resulting waste inside
202. 8:46the cabinet with a loss of cabinet power
203. 8:49or other emergency that requires
204. 8:50immediate attention or evacuation follow
205. 8:54the same basic steps quickly secure
206. 8:57biological material remove PPE and step
207. 9:01away from the situation no matter what
208. 9:03the situation remain calm
209. 9:06seek help from a co-worker when you need
210. 9:08it and most of all always remember that
211. 9:10your own safety comes first having
212. 9:14emergency procedures in place for
213. 9:16biosafety cabinet work will help
214. 9:18laboratory operators prepare for the
215. 9:20unexpected thank you for your time and
216. 9:22remember stay safe
217. 9:25[Music]
218. 9:27you