Biological safety cabinet (BSC) 3: Best practices for safe usage — Transcript

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1. 0:03[Music]
2. 0:05it is always important to follow best
3. 0:08practices while working in a biosafety
4. 0:10cabinet also known as a BSC these
5. 0:14practices will help maintain adequate
6. 0:16airflow within the cabinet to help
7. 0:19prevent exposures and release of
8. 0:20pathogens they should also be
9. 0:22incorporated into your facility's
10. 0:24standard operating procedures now let's
11. 0:28take a look at some of these recommended
12. 0:29practices work in a BSC should be
13. 0:32carried out by one person at a time all
14. 0:35work in a BSC should be carried out as
15. 0:38far as possible towards the rear of the
16. 0:40work area elbows and arms should not
17. 0:43rest on the grill or work surface unless
18. 0:46the BSC has been outfitted with
19. 0:48appropriate arm rests one should avoid
20. 0:51quick and excessive movement of hands
21. 0:53and arms through the front opening such
22. 0:55movements disrupt the air curtain at the
23. 0:58front of the BSC
24. 0:59which can allow contaminants to enter or
25. 1:01escape arms should enter and exit the
26. 1:04BSC slowly and perpendicular to the
27. 1:07front opening a bottle of an appropriate
28. 1:10disinfectant should be kept within the
29. 1:12BSC while work is being performed this
30. 1:16will reduce the need to remove your
31. 1:17hands from the BSC it is recommended to
32. 1:21segregate non contaminated items also
33. 1:24known as clean items from contaminated
34. 1:27items also known as dirty items work
35. 1:30should always flow from clean to dirty
36. 1:32areas material should be discarded in a
37. 1:36waste container that is located towards
38. 1:38the side of the cabinet workspace do not
39. 1:42discard materials in containers outside
40. 1:44of the cabinet open flames such as
41. 1:47Bunsen burners should not be used inside
42. 1:50a BSC as they create turbulence disrupt
43. 1:53airflow patterns and can damage the HEPA
44. 1:56filter if absolutely necessary touch
45. 2:00activated micro burners are preferred to
46. 2:02reduce airflow disturbance non flame
47. 2:06alternatives such as sterile disposable
48. 2:08inoculation loops
49. 2:10be used whenever possible equipment
50. 2:13creating air movement such as vacuum
51. 2:15pumps and centrifuges may affect the
52. 2:18integrity of the airflow and should not
53. 2:20be used within the BSC and finally
54. 2:23windows should be kept closed and
55. 2:25cooling fans should be turned off when
56. 2:27the BSC is in use in order to minimize
57. 2:30disruptions to the air movement once you
58. 2:33have completed your work you still have
59. 2:35a few additional procedures to follow
60. 2:37before turning off the BSC containers
61. 2:41should be closed or covered and all
62. 2:43items should be surface decontaminated
63. 2:46before removing them from the BSC
64. 2:48disinfect the interior surfaces of the
65. 2:51BSC including sides back and interior of
66. 2:55the glass use a disinfectant that is
67. 2:58effective against the pathogens in use
68. 3:00allowing an appropriate contact time if
69. 3:03a corrosive disinfectant is used the
70. 3:06surface should be wiped down after
71. 3:08disinfection and sprayed with 70%
72. 3:10ethanol to avoid corrosion of the
73. 3:13stainless steel surfaces one should also
74. 3:16routinely remove the work surface and
75. 3:18disinfect the tray beneath you should
76. 3:21also allow sufficient time for the air
77. 3:23in the BSC to purge before disrupting
78. 3:26the air curtain by removing hands or
79. 3:28unloading material from the BSC the
80. 3:31purge time will vary by model and can be
81. 3:34up to several minutes the use of
82. 3:36ultraviolet UV germicidal lamps to
83. 3:39disinfect a contaminated BSC is strongly
84. 3:42discouraged due to their limited
85. 3:44effectiveness at disinfecting the inside
86. 3:47of BSC s UV irradiation of the work area
87. 3:51should only be used as a secondary
88. 3:53method of disinfection in the cabinet
89. 3:56let's conclude this video with a review
90. 3:59of the following key messages a facility
91. 4:02standard operating procedures should
92. 4:04include best practices that minimize the
93. 4:06disruption of air flow while working in
94. 4:09a BSC
95. 4:10work from clean to dirty and dispose of
96. 4:13contaminated waste inside the cabinet it
97. 4:16is important to decontaminate items and
98. 4:19surfaces within the BSC after completing
99. 4:22your work do not rely on UV irradiation
100. 4:26alone to disinfect a contaminated work
101. 4:29area knowledge of how to work properly
102. 4:32in a BSC is a key component to working
103. 4:35safely when using them
104. 4:36[Music]
105. 4:38you