Biological safety cabinet (BSC) 2: Preparatory steps — Transcript

Full transcript

1. 0:00[Music]
2. 0:06biosafety cabinets are primary
3. 0:08containment devices designed to protect
4. 0:10laboratory workers from potential
5. 0:12exposure to infectious agents and the
6. 0:15release of the agents to the surrounding
7. 0:16environment prior to working with
8. 0:19infectious material inside the biosafety
9. 0:21cabinet there are a few important steps
10. 0:24to take to make sure that work will be
11. 0:26done safely and the cabinet is
12. 0:27functioning correctly every institution
13. 0:30should develop a standard operating
14. 0:32procedure or SOP for the correct use of
15. 0:35biosafety cabinets the SOP should
16. 0:38contain introductory information on BSC
17. 0:41s outline the risk assessment process
18. 0:43for selecting the most appropriate
19. 0:45cabinet outline staff training
20. 0:48requirements and provide detailed
21. 0:50information on all steps required for
22. 0:52the safe use of this equipment before
23. 0:55using a biosafety cabinet laboratory
24. 0:58staff should verify the most recent
25. 1:00certification date recertification is
26. 1:03ideally done on an annual basis however
27. 1:05many parts of the world lack trained
28. 1:08technicians and equipment resulting in
29. 1:10BSC s that are serviced less frequently
30. 1:12during periods between recertification
31. 1:16there are a few simple ways that
32. 1:17laboratory staff can perform qualitative
33. 1:20assessments of the error curtain and
34. 1:21function of the HEPA filter holding a
35. 1:24lightweight tissue paper and moving over
36. 1:26the areas immediately in front of the
37. 1:29opening the paper should always be
38. 1:31pulled inward this provides a visual
39. 1:33confirmation that the air curtain is
40. 1:35being maintained a similar procedure can
41. 1:38be performed inside the cabinet by
42. 1:40grasping a tissue paper with a pair of
43. 1:42tweezers and moving it around the
44. 1:44cabinet to observe airflow patterns if
45. 1:47unexplained contamination has become a
46. 1:50recurring problem the HEPA filter
47. 1:52responsible for delivering sterile air
48. 1:54over the work surface could also be a
49. 1:56contributing factor a damaged or leaky
50. 1:59supply HEPA can blow unfiltered air over
51. 2:02the work surface as a qualitative test
52. 2:05place open agar plates in the biosafety
53. 2:08cabinet for a period of time and check
54. 2:10for bacterial growth following
55. 2:12incubation
56. 2:13the presence of colonies on the plate
57. 2:15could indicate a serious problem with
58. 2:17the BSC however it's important to
59. 2:20remember that not everything the heba
60. 2:22serves to filter out will grow on an
61. 2:24agar plate and lack of growth does not
62. 2:27guarantee that the filter is functioning
63. 2:29as required for these reasons
64. 2:32recertification by trained technicians
65. 2:34with the appropriate equipment is the
66. 2:36only way to ensure that the critical
67. 2:38components of a biosafety cabinet are
68. 2:40functioning as required the first step
69. 2:43before using a biosafety cabinet is
70. 2:45turning on the blower allow sufficient
71. 2:48time for the air to purge and the
72. 2:50pressure reading to stabilize before
73. 2:52initiating work this will be specified
74. 2:55in the manufacturer's instructions and
75. 2:57is generally three to five minutes
76. 2:59ensure that the air intake and exhaust
77. 3:02grills are free from obstructions and
78. 3:04confirm inward airflow with a tissue
79. 3:07prior to each use based on your local
80. 3:10risk assessment additional personnel
81. 3:12protective equipment or PPE may be
82. 3:15required for the work you'll be
83. 3:16performing tuck the cuffs of your gown
84. 3:19or lab coat into your gloves to reduce
85. 3:21the likelihood of having exposed skin
86. 3:24lab coats with elastic cuffs are
87. 3:26preferred over those with standard
88. 3:28sleeves as there is less of a chance
89. 3:30they will slip out of your gloves and
90. 3:31potentially come in contact with your
91. 3:33work next clean the interior surface of
92. 3:37the cabinet with 70% ethanol or other
93. 3:39appropriate disinfectant wear gloves to
94. 3:42protect your skin once the BSC is clean
95. 3:46work materials may be placed inside to
96. 3:49facilitate clean up later on plastic
97. 3:51packed absorbent towel may be placed on
98. 3:53the work surface disinfect items with
99. 3:5670% ethanol before placing them inside
100. 3:59to avoid introducing potential
101. 4:01contaminants into the cabinet try to
102. 4:04bring in all the materials you'll need
103. 4:05without overcrowding the cabinet and
104. 4:08ensure adequate supplies for elimination
105. 4:10of waste and disinfection while working
106. 4:13while 70% ethanol is highly effective
107. 4:16against many pathogens a
108. 4:19assessment will help you select the best
109. 4:20disinfectant to keep on hand as a
110. 4:23practical example will prepare the
111. 4:25cabinet for processing patient samples
112. 4:27for PCR and other downstream testing
113. 4:30arrange the items inside the BSC in a
114. 4:33logical order that allows work from
115. 4:35clean to dirty roughly dividing the
116. 4:38cabinet workspace into three sections
117. 4:40keep clean items towards one outer end
118. 4:43designate the middle third of the
119. 4:45cabinet as a work zone and keep waste
120. 4:47receptacles at the other end aerosol
121. 4:50generating equipment such as vortex
122. 4:52mixers should be placed as far to the
123. 4:55rear of the cabinet as possible
124. 4:57taller items such as biohazard bags
125. 5:00should be placed towards the side walls
126. 5:02to minimise airflow disruption for this
127. 5:05reason trays for contaminated
128. 5:06serological pipettes are preferred over
129. 5:09vertical waste receptacles next place
130. 5:12the biological materials inside the
131. 5:15cabinet once everything is inside the
132. 5:17BSC adjust the chair height so that
133. 5:20armpits are level with the sash opening
134. 5:22you are now ready to begin your work
135. 5:25let's conclude this video with a review
136. 5:28of the following key messages ensure BSC
137. 5:32s are adequately serviced and maintained
138. 5:34prior to use don appropriate PPE
139. 5:38disinfect the inside of the cabinet and
140. 5:40the surface of all items placed inside
141. 5:43organise materials to promote workflow
142. 5:46from clean to dirty keep a supply of
143. 5:49disinfectant and cleaning materials
144. 5:51inside the cabinet safe use of a BSC
145. 5:55begins even before sitting at the
146. 5:57cabinet for work planning ahead and
147. 5:59proper BSC setup will allow you to work
148. 6:02safely and more efficiently
149. 6:04[Music]
150. 6:05you